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OJVRTM
Online Journal of
Veterinary Research©
Volume 20(4):243-253, 2016.
Dichelobacter nodosus and Fusibacterium necrophorum
virulence
in cross infected
foot rot of sheep and cattle.
Mohammad
Mostafa Nazaria*, Abbas Barinb, Mehran Farhoodi
Moghadamc,
aDepartment of Microbiology and Cell research, cClinical
Sciences, Faculty of Veterinary Medicine, Karaj Branch, Islamic Azad
University, Alborz, bDepartment of
Microbiology, Faculty of Veterinary Medicine, University of Tehran, Tehran,
ABSTRACT
Nazari MM, Barin A, Moghadam MF, Dichelobacter nodosus and Fusibacterium necrophorum virulence in cross infected foot rot of sheep
and cattle, Onl J Vet Res., 20(4):243-253, 2016. Quantitative PCR assay to determine D. nodosus and F. necrophorum
loads in ruminant foot-rot is described. Dichelobacter nodosus isolates from foot rot in sheep and cattle were
subjected to virulence test fimA, serogrouping
and pulsed field gel electrophoresis (PFGE). Virulent D. nodosus
were serogroup B, intA positive and aprB2 negative. F.
necrophorum was found more frequently in feet
contaminated by virulent D. nodosus. F. necrophorum subsp. necrophorum
were detected in cross-infected foot rot and greater loads of virulent D. nodosus and F. necrophorum
were found in severe foot rot in sheep grazing with cattle. D. nodosus loads were greater with interdigital dermatitis
and severe foot rot but were also isolated from normal animals. Multiple
strains (serogroup) of D. nodosus co-existed
on individual feet during subclinical and clinical infections.
Keywords:
Dichelobacter nodosus;
Fusobacterium necrophorum; cross-infection;
serogroup analysis, virulent; environment.
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