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Online Journal of Veterinary Research

(Including Medical and Laboratory Research)

Established 1994
ISSN 1328-925X


Volume 26 (12): 874-881, 2022.

Effect of sodium nitrite on arteries of adult male and female rats.


Fatemeh Juibar PhD 1, Saeed Khatamsaz PhD2.


1 Young Researchers and Elite Club, 2Biology, Kazerun Branch, Islamic Azad University, Iran.



Juibar F, Khatamsaz S., Effect of sodium nitrite on arteries of adult male and female rats, Onl J Vet Res., 26 (12): 874-881, 2022. Nitrates and nitrites are used as food preservatives but at high doses may induce gastrointestinal cancer and methemoglobinemia in infants. To our best knowledge effects of this preservatives on arteries has not been determined. Three groups of 10 female and male 200-220g Albino Wistar rats each were kept in cages at 25-27C and 12h light/dark cycle, fed pellets and water ad libitum acclimatized 2 weeks. Then, rats were given 175 (1) or 350mg/kg (2) daily for 60 days in drinking water. Controls (3) were not treated. Body weights were measured daily. In anaesthetized rats, at day 60, we measured nitric oxide (μM/L) from cardiac blood by Griess Method. After sacrifice, aortic lung, kidney, carotid and abdominal arteries were dissected preserved in 31% formalin (31%) for microscopy with Hematoxilin-Eosin and Mason Tri chrome stains. Compared with controls, in rats given sodium nitrite, we found ~10 to 16 fold increase in plasma nitric oxide levels but no differences between genders. High dose 350mg/kg sodium nitrite reduced width of tunica media in all arteries ~18 to 21% (P < 0.05) with lymphocyte infiltrate and inflammation. We found no effect of 175mg/kg nitrite on arteries


Key word: sodium nitrite, artery, rat, nitric oxide.