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OJVRTM
Online Journal
of Veterinary Research©
(Including Medical and Laboratory Research)
Established 1994
ISSN 1328-925X
Volume 26 (12): 874-881, 2022.
Effect of sodium nitrite on arteries of adult male and female rats.
Fatemeh
Juibar PhD 1, Saeed Khatamsaz PhD2.
1 Young Researchers and
Elite Club, 2Biology, Kazerun Branch, Islamic Azad University, Iran.
ABSTRACT
Juibar F, Khatamsaz S., Effect of sodium
nitrite on arteries of adult male and female rats, Onl
J Vet Res., 26
(12): 874-881, 2022. Nitrates and nitrites are used as food preservatives but at high doses
may induce gastrointestinal cancer and methemoglobinemia
in infants. To our best knowledge effects of this preservatives on arteries has
not been determined. Three groups of 10 female and male 200-220g Albino Wistar rats each were kept in cages at 25-27°C and 12h
light/dark cycle, fed pellets and water ad
libitum acclimatized 2 weeks. Then, rats were given 175 (1) or 350mg/kg (2)
daily for 60 days in drinking water. Controls (3) were not treated. Body
weights were measured daily. In anaesthetized rats, at day 60, we measured
nitric oxide (μM/L) from cardiac blood by Griess Method. After sacrifice, aortic
lung, kidney, carotid and abdominal arteries were dissected preserved in 31%
formalin (31%) for microscopy with Hematoxilin-Eosin
and Mason Tri chrome stains. Compared with controls, in rats given sodium
nitrite, we found ~10 to 16 fold increase in plasma nitric oxide levels but no
differences between genders. High dose 350mg/kg sodium nitrite reduced width of
tunica media in all arteries ~18 to 21% (P < 0.05) with lymphocyte infiltrate and inflammation. We found no effect of 175mg/kg nitrite on
arteries
Key word: sodium nitrite, artery, rat, nitric oxide.