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OJVRTM
Online Journal of Veterinary
Research©
25 (3): 252-258,
2021.
Effect of N-nitro-L-arginine
methyl ester dihydrochloride on progesterone, estrus and embryos of buffalo.
Chetna Gangwar1, Shiv Prasad2, JK Prasad3,
SP Singh4 and AK Verma5
1, 2 and 3Department of
Animal Reproduction, Gynaecology and Obstetrics, CVSc,G. B. Pant University of Agriculture and Technology, Pantnagar, 4Department of Anatomy, DUVASU,
Mathura, India,5 Dept of Epidemiology and Veterinary Preventive Medicine, DUVASU,
Mathura, India.
ABSTRACT
Gangwar C, Prasad S, Prasad JK, Singh SP, Verma AK., Effect of N-nitro-L-arginine methyl ester dihydrochloride on estrus and
embryos of buffalo, Onl J Vet Res., 25 (3): 252-258, 2021.
Authors report effect of nitric oxide (NO) synthase
inhibitor, N-nitro-L-arginine methyl ester dihydrochloride
(L-NAME) on blood progesterone, NO and estrus. Groups
of 8 cycling buffaloes each were injected 25mg PGF2α IM to
induce luteolysis and estrus.
Eighty controls were injected saline. Saline or L-NAME was then given through catheterized
jugular veins at day 14 of estrus. On day 15 of estrus, controls were infused saline and treated buffalo
infused 400 µg/hour L NAME (total 800 µg). Estrus was
assessed by vaginal discharge, mounting and rectal examination at 6h intervals
24h after PGF2α. Blood was
taken at 4h interval on day 15 to 16 and once from day 17 to 21 of estrus. Progesterone was determined by radioimmunoassay and
nitrate metabolites by calorimetric Griess reaction. Inhibition
of NO was evaluated by prostaglandin (PGF2α)
induced regression of corpus luteum. L-NAME prolonged estrus
~9% in buffalo given L-NAME compared (P<0.05) with controls 20.12±0.13. By
rectal examination, CL was present for longer duration in treated groups. L-NAME
increased (P<0.05) serum progesterone by ~47% peaking at Day 15. Serum
nitrate/nitrite was ~25% lower (P < 0.05) in ruminants given L NAME by day
21 (4.80±029 versus 3.61±µg/ml). Results suggest L-NAME stimulated progesterone
secretion (P<0.05), inhibited nitrates and extended estrus
(P<0.05). We find that L-NAME could minimize early embryonic losses due to
luteal insufficiency in buffaloes.
Key
words: Buffaloes, Corpus luteum function,
Nitric oxide, NOS inhibitor (L-NAME), Ovulatory cycle, Progesterone (P4).
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