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Online Journal of Veterinary Research


25 (3): 252-258, 2021.

Effect of N-nitro-L-arginine methyl ester dihydrochloride on progesterone, estrus and embryos of buffalo.


Chetna Gangwar1, Shiv Prasad2, JK Prasad3, SP Singh4 and AK Verma5


1, 2 and 3Department of Animal Reproduction, Gynaecology and Obstetrics, CVSc,G. B. Pant University of Agriculture and Technology, Pantnagar, 4Department of Anatomy, DUVASU, Mathura, India,5 Dept of Epidemiology and Veterinary Preventive Medicine, DUVASU, Mathura, India.




Gangwar C, Prasad S, Prasad JK, Singh SP, Verma AK., Effect of N-nitro-L-arginine methyl ester dihydrochloride on estrus and embryos of buffalo, Onl J Vet Res., 25 (3): 252-258, 2021. Authors report effect of nitric oxide (NO) synthase inhibitor, N-nitro-L-arginine methyl ester dihydrochloride (L-NAME) on blood progesterone, NO and estrus. Groups of 8 cycling buffaloes each were injected 25mg PGF IM to induce luteolysis and estrus. Eighty controls were injected saline. Saline or L-NAME was then given through catheterized jugular veins at day 14 of estrus. On day 15 of estrus, controls were infused saline and treated buffalo infused 400 g/hour L NAME (total 800 g). Estrus was assessed by vaginal discharge, mounting and rectal examination at 6h intervals 24h after PGF. Blood was taken at 4h interval on day 15 to 16 and once from day 17 to 21 of estrus. Progesterone was determined by radioimmunoassay and nitrate metabolites by calorimetric Griess reaction. Inhibition of NO was evaluated by prostaglandin (PGF) induced regression of corpus luteum. L-NAME prolonged estrus ~9% in buffalo given L-NAME compared (P<0.05) with controls 20.120.13. By rectal examination, CL was present for longer duration in treated groups. L-NAME increased (P<0.05) serum progesterone by ~47% peaking at Day 15. Serum nitrate/nitrite was ~25% lower (P < 0.05) in ruminants given L NAME by day 21 (4.80029 versus 3.61g/ml). Results suggest L-NAME stimulated progesterone secretion (P<0.05), inhibited nitrates and extended estrus (P<0.05). We find that L-NAME could minimize early embryonic losses due to luteal insufficiency in buffaloes.


Key words: Buffaloes, Corpus luteum function, Nitric oxide, NOS inhibitor (L-NAME), Ovulatory cycle, Progesterone (P4).