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OJVR
Online Journal of Veterinary Research©
Volume 14 (1):49-55, 2010. Extensively redacted 2017.
Effect of
nitric oxide (NO) synthase inhibitor on progesterone secretion, NO
concentration and estrous cycle length in buffaloes
Chetna Gangwar1, Shiv Prasad2, JK
Prasad3, SP Singh4 and AK Verma5#
1,2, 3Department of
Animal Reproduction, Gynaecology and Obstetrics, CVSc, G. B. Pant University of Agriculture and Technology, Pantnagar, 4 Department(s) of Anatomy and 5Epidemiology and Veterinary Preventive Medicine, DUVASU, Mathura, India.
ABSTRACT
Gangwar C, Prasad S, Prasad JK, Singh SP,
Verma AK., Effect of nitric oxide (NO) synthase
inhibitor on progesterone secretion, NO concentration and estrous
cycle length in buffaloes, Onl J Vet Res, 14
(1):49-55, 2010. Groups of 8
cycling buffaloes were injected IM 25 mg PGF2α during the
luteal phase to induce luteolysis and estrus. On day 15 of estrus, 8
controls were infused saline and those given PGF2a were infused 400 µg/h L-NAME
nitric oxide synthatase inhibitor. Blood samples were
collected every 4 h on day 15 to day 16 and once per day 17 tol
day 21. Progesterone was determined by RIA kit, Nitrate/nitrite stable
metabolites of NO by calometry. Compared with
controls, results suggest that L-NAME stimulated progesterone secretion, decreased serum nitrate/nitrite and extended
functional life of the corpus luteum. Nitric
oxide inhibitor could be used to minimize early embryonic losses due to
luteal insufficiency in buffaloes.
Key words:
Buffaloes, Corpus luteum function, Nitric oxide, NOS inhibitor (L-NAME),
Ovulatory cycle, Progesterone (P4).