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Online Journal of Veterinary Research

Volume 24 (1):15-27, 2020.

..Effect of nitric oxide compounds on lymphocyte proliferation in high and low milk producing Murrah buffalo.


Joydip Mukherjee, Sujata Pandita, Ruokuobeinuo Huozha, Vijay Kumar and Manju Ashutosh.


Dairy Cattle Physiology Division, NDRI, Karnal, India.




Mukherjee J, Pandita S, Huozha R, Kumar V, Ashutosh M., Effect of nitric oxides on lymphocyte proliferation in high and low milk producing Murrah buffalo, Onl J Vet Res., 24 (1):15-27, 2020. Groups of 6 Murrah buffalo each of similar parity at 90-120 days lactation were selected for higher (9.3L/Day) or lower milk (6L/Day) production. Blood was taken every 10 days for 30 days. Lymphocytes were isolated and cultured in RPMI 1640 medium with PHA-P for 24h at 37oC in a humidified CO2 incubator (95% air and 5% CO2). Lymphocyte responsiveness in vitro to S-Nitroso-N-acetylpenicillamine (S-NAP) nitric oxide donor or N-nitro-L-arginine methyl ester (L-NAME) and L-Mono-methyl-arginine (L-NMMA) NO synthetase inhibitors were assessed. Mitogen induced stimulation index was higher (P<0.01) in buffaloes with higher milk production. S-NAP at 10mM reduced (P < 0.01) lymphocyte blastogenesis ~20% in both groups whereas only 0.2mM L-NAME and NMMA had similar effect (P<0.01).


Key-words: Nitric oxide, blastogenesis, milk, buffalo.