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OJVRTM
Online Journal of
Veterinary Research©
Volume
24 (1):15-27, 2020.
..Effect
of nitric oxide compounds on lymphocyte proliferation in high and low milk
producing Murrah buffalo.
Joydip Mukherjee, Sujata Pandita, Ruokuobeinuo Huozha, Vijay Kumar
and Manju Ashutosh.
Dairy Cattle Physiology Division, NDRI, Karnal,
India.
ABSTRACT
Mukherjee
J, Pandita S, Huozha R,
Kumar V, Ashutosh M., Effect of nitric oxides on lymphocyte proliferation in high and low milk
producing Murrah buffalo, Onl
J Vet Res., 24 (1):15-27, 2020. Groups of 6 Murrah
buffalo each of similar parity at 90-120 days lactation were selected for higher
(9.3L/Day) or lower milk (6L/Day) production. Blood was taken every 10 days for
30 days. Lymphocytes were isolated and cultured in RPMI 1640 medium with PHA-P
for 24h at 37oC in a humidified CO2 incubator (95% air
and 5% CO2). Lymphocyte responsiveness in vitro to S-Nitroso-N-acetylpenicillamine (S-NAP) nitric oxide donor or
N-nitro-L-arginine methyl ester (L-NAME) and L-Mono-methyl-arginine (L-NMMA) NO
synthetase inhibitors were assessed. Mitogen induced
stimulation index was higher (P<0.01) in buffaloes with higher milk
production. S-NAP at 10mM reduced (P < 0.01) lymphocyte blastogenesis
~20% in both groups whereas only 0.2mM L-NAME and NMMA had similar effect
(P<0.01).
Key-words:
Nitric oxide, blastogenesis, milk, buffalo.
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