©2023-2035 All Rights Reserved. Online Journal of Veterinary Research.  You may not store these pages in any form except for your own personal use. All other usage or distribution is illegal under international copyright treaties. Permission to use any of these pages in any other way besides the before mentioned must be gained in writing from the publisher. This article is exclusively copyrighted in its entirety to onlinejournals@gmail.com publications.. This article may be copied once but may not be, reproduced or  re-transmitted without the express permission of the editors. Linking: To link to this page or any pages linking to this page you must link directly to this page only here rather than put up your own page.             

                                                                                                                                                                                                                                                                                                                                                                                                                                                           

OJVRTM

 

Online Journal of Veterinary Research©

(Including Medical and Laboratory Research)

Established 1994

ISSN 1328-925X

 

Volume 28 (8): 490-496, 2024.

Effect of nitro-arginine on bovine progesterone and oestrus.

 

Chetna Gangwar, Shiv Prasad, JK Prasad, SP Singh, AK Verma.

 

Department of Animal Reproduction, Gynaecology and Obstetrics, University of Agriculture and Technology, Pantnagar, Department of Anatomy, Dept of Epidemiology and Veterinary Preventive Medicine Mathura, India.

 

ABSTRACT

 

Gangwar C,  Prasad S, Prasad JK, Singh SP, Verma AK., Effect of nitro-arginine on bovine progesterone and oestrus, Onl J Vet Res., 28(8): 490-496, 2024. We report effect of N-nitro-L-arginine methyl ester (L-NAME) on serum progesterone, nitric oxide and oestrus in buffaloes. Eight cycling 4-8 years old buffaloes were injected 25mg/kg L-NAME to induce luteolysis and oestrus. Another 8 controls were infused saline. On day 15 of oestrus, buffalo were infused 400 µg/hour L-NAME for 2h in 500 ml normal saline. Oestrus was detected by mounting, mucous discharge and rectal examination at 6h intervals after 24h PGF2α. Blood was taken at 4h intervals on day 15 to day 16 and once daily at days 17 to day 21 of oestrus. Serum progesterone was determined by radioimmunoassay and nitrate/nitrite by calorimetry. Administration of L-NAME enhanced (P<0.05) oestrus ~2 days, serum progesterone ~47% and nitrate/nitrites ~25% compared with controls.

 

Key words: Buffaloes, Corpus luteum function, Nitric oxide, NOS inhibitor (L-NAME), Ovulatory cycle, Progesterone (P4).