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Online Journal of Veterinary Research©
Volume 22 (7):602-607, 2018.
Effect of Nisin on MCF-7 tumor cell viability and apoptosis in RPMI media.
Ghorbankhani Abbas1, Mohammadi Ali1, Kazemipour Nasrin2, Hashempour Sadeghian Mahdi2
1Department(s) of Pathobiology, 2Basic Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran
Abbas G, Ali M, Nasrin K, Mahdi HS, Effect of Nisin on MCF-7 tumor cell viability and apoptosis in RPMI media, Onl J Vet Res., 22 (7):602-607, 2018. MCF-7 breast tumor cells were cultured in RPMI 1640 for 24 hours and treated with 20, 40 and 80 µg/ml nisin for 24, 48 and 72 hours. Results were compared against MCF-7 cells in RPMI controls. Cultured cells were analyzed by MTT, trypan blue and acridine orange/ethidium bromide (AO/EB) assays. There was no change in percentage of apoptotic cells in control samples. By MTT assay we found decreased (P < 0.05) viability of cells in 20 µg/ml nisin to 72 hours whereas those in 40 µg/ml, only to 48 hours. In 80 µg/ml cell viability decreased (P <0.05) at 24 hours but did not change by 48 or 72 hours. At 24 hours cell viability in 80 µg/ml was lowest but in 20 µg/ml highest. However, After 72 hours exposure at any dose of nisin we found no difference in live cell numbers. By Trypan Blue assay, cell viability declined greatly (P < 0.001) in 20 and 40 µg/ml nisin but most in 80µg/ml nisin at 24, 48 or 72 hours. By AO/EB assay we found time-dose increased effect of nisin (P < 0.05) on percentage of apoptotic cells. Our findings suggest that nisin reduces viability and increases apoptosis of MCF-7 cell line. However type of assay appears to affect results.
Keywords: Nisin, Breast cancer, MCF-7, antimicrobial peptide.