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OJVRTM
Online Journal of
Veterinary Research©
Volume 21(10):657-668, 2017.
In
situ PCR for detection and differentiation of Newcastle disease virus strains
in Leghorn chickens
Elawad A Hussein1,
M Hair-Bejo1,2*, AR Omar1,2, SS Arshad1
and I Aini1,2
1Department of Veterinary Pathology and
Microbiology, Faculty of Veterinary Medicine, 2Institute of Bioscience, Universiti Putra
Malaysia, Serdang, Selangor, Malaysia *Correspondence: mdhair@upm.edu.my
ABSTRACT
Hussein EA, Hair-Bejo M, Omar AR, Arshad
SS, AiniI., In situ PCR for detection and
differentiation of Newcastle disease virus strains in leghorn chickens, Onl J Vet Res., 21(10):657-668, 2017. In experiment
1, six specific pathogen free (SPF) chickens were intra-nasally infected with velogenic (v) NDV strain with titre of 10⁵ EID50/0.1
mL and 6 non-infected birds were used as controls. Chickens were sacrificed at
different times and tissue samples were collected for In situ PCR and immunoperoxidase staining
(IPS). In situ PCR was more
sensitive (P < 0.05) than IPS for detection of NDV. In a 2nd
experiment, In situ PCR was
done to differentiate NDV strains. Groups of 5 SPF chickens each, were infected
with velogenic (10⁵EID50/0.1 mL) or lentogenic (l) NDV (103.0 EID50/0.1
mL) strains. Non-infected birds were used as controls. After sampling of
tissues, an In situ PCR was developed
using specific velogenic and lentogenic
strain probes. In situ PCR velogenic probe was positive only to tissues infected by velogenic strain whereas lentogenic
probe only with lentogenic infected-tissues. The
findings suggested that the In situ PCR differentiated lentogenic
from velogenic NDV virus strains.
Keywords: In situ PCR, Newcastle disease virus strains, lentogenic, velogenic.
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