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Online Journal of Veterinary Research


Volume 26 (8): 593-598, 2022.

Seroprevalence of Newcastle disease antibodies in vaccinated broilers and layers.


Ali Mahdi Sahib1, Khaleel Z Al-Alo2, Hayder R Abed3, Kaveh Veisi4


1, 3Department of Public Health, 2Clinical Sciences, College of Veterinary Medicine, University of Kufa, Iraq

4Faculty of Veterinary Medicine, University of Tehran, Iran




Sahib AM, Al-Alo KZ, Hayder RA, Veisi K, Seroprevalence of Newcastle disease antibodies in vaccinated broiler and layers, Onl J Vet Res, 26 (8): 593-598, 2022. We report effect of vaccination on Newcastle virus antibody titers under field conditions in 55 broilers and layers. Birds were vaccinated alpha-herpesvirus, Massachusetts with or without Lasota, Lukert and pox, subcutaneously, intramuscular, by spray or drinking water at 1 to 119 days of age. Wing vein blood was aspirated at 30 days in broilers and 140 days for serum Newcastle virus antibody detected by ELISA assay. We found 71% prevalence in broilers and 91% in layers with no significant difference between them. However mean antibody titers in layers were 33890.3 930.3 but in broilers only 1407.6 31.7, a 24 fold difference (P < 0.01). Maximum titers in broiler were 1736 and in layers 43218 (P < 0.05). Results suggest that age and repeat vaccinations in layers account for much higher titer response to Newcastle virus.


Key words: Newcastle disease, Vaccination, antibody titer, ELISA test, chickens.