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OJVRTM

Online Journal of Veterinary Research©

Volume 19(9): 689-697, 2015.

 

 

Effect of inactivated Neospora caninum tachyzoite vaccine on immune markers

and transplacental transmission in pregnant BALB/c mice.

 

Abdeyazdan A1, Namavari M2*

 

1Islamic Azad University, 2Kazerun Razi Vaccine and Serum Research Institute, Shiraz, Iran

 

ABSTRACT

 

Abdeyazdan A, Namavari M., Effect of inactivated Neospora caninum tachyzoite vaccine on immune markers and transplacental transmission in pregnant BALB/c mice, Onl J Vet Res., 19(9): 689-697, 2015. Neospora caninum causes abortion in cattle.  Neospora vaccines can minimize and control the infection in mice but not in cattle. Eight mice were subcutaneously injected 30-70% water-in-Montanide ISA 773 oil emulsions with 1 × 106 inactivated tachyzoites. After immunization and mating, pregnant mice were challenged with live 2×106 tachyzoites. Parasites present in the brain and tissues of the pups and adult mice were detected/assessed by PCR, serological and footpad tests. Vaccinated adults or pups showed no symptoms of neosporosis until euthanasia whereas un-vaccinated oil controls (8) showed severe weight loss and posterior limb paralysis. Four of eight plugged mice in the vaccinated group gave birth with lower pregnancy rate compared with controls. Antibody against N. caninum was detected in all mice vaccinated with whole killed tachyzoite compared with mice in control group. Vaccinated mice showed significant footpad swelling whereas controls did not and parasite DNA was detected in brain tissue of all controls but none in vaccinated mice.

 

Keywords: Neospora caninum,   Tachyzoite, Vertical transmission, Mouse, Oil adjuvant.

 

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