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OJVRTM
Online
Journal of Veterinary Research©
Volume 16 (1):38-48, 2012. Redacted
2017.
Effect of host cell on virulence of Neospora
caninum
Khordadmehr,
M.1, Khodakarm-Tafti, A. 1,
Namavari, M2*, Mansourian,
M.2, Karimiyan, A.3, Rahimian, A2.
1. Department of Pathology, School of
Veterinary Medicine, Shiraz University, 2. Razi
Vaccine and Serum Research Institute, Shiraz,3.
Islamic Azad University of Jahrom, Jahrom, Iran
ABSTRACT
Khordadmehr
M, Khodakarm-Tafti A, Namavari
M, Mansourian M, Karimiyan
A, Rahimian A., Effect of host cell on virulence of Neospora caninum, Online J Vet
Res, 16(1):38-48, 2012. Neospora
caninum causes major bovine abortions and
neonatal mortality in cattle, sheep, goat and horse. Vero cells are the most
frequently used cell line for culture of Neospora
caninum but
problems with propagation and required level of nutritional components for the
media persist. Attenuation of virulence after 3 months continuous passages of
suspension culture of Theileria lestoquardi-infected lymphoblastoid
(TLI) cell line was compared with Vero cell line. Different doses of N. caninum tachyzoites harvested
from these cell lines were inoculated into embryonated
broiler eggs and daily mortality rate, pathological and molecular effects were
recorded. Continuous passage of Neospora
caninum in both cell lines were similar
suggesting that TLI cells could be used for vaccine production.
Keywords: Neospora caninum, Vero
cell line, T. lestoquardi-infected lymphoblastoid cell line
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