©1996-2018 All Rights Reserved. Online
Journal of Veterinary Research. You may not store these pages in any form
except for your own personal use. All other usage or distribution is illegal
under international copyright treaties. Permission to use any of these pages in
any other way besides the before mentioned must be gained in writing from the
publisher. This article is exclusively copyrighted in its entirety to OJVR.
This article may be copied once but may not be, reproduced or re-transmitted
without the express permission of the editors. This journal satisfies the
refereeing requirements (DEST) for the Higher Education Research Data
Collection (Australia). Linking: To link to this page or any pages linking to
this page you must link directly to this page only here rather than put up your
own page
OJVRTM
Online Journal of
Veterinary Research©
Volume 21(8):505-510, 2017.
Myostatin (gdf-8)
polymorphism and its association with growth traits in Baluchi sheep
Ali akbar Masoudi1*, Abdolrahim Abbasi2 ,Hossein Emrani2, Saeid Esmaeilkhanian2 and Sima
Savar Sofla2.
1Department of Animal Science, Faculty of
Agriculture, Tarbiat Modares University, Tehran, 2Animal Science
Research Institute of Iran, Agricultural Research, Education and Extension
Organization (AREEO), Karaj, Iran
ABSTRACT
Masoudi AA, Abbasi A,
Emrani H, Esmaeilkhanian S, Sofla SS., Abbasi A., Myostatin (gdf-8) polymorphism
and its association with growth traits in Baluchi sheep, Onl J Vet Res., 21(8):505-510,
2017. Myostatin
represses skeletal muscle growth, but mutations can produce supernumerary
muscle fibers, and increase muscle mass. Authors describe association of
myostatin polymorphisms with growth traits in Baluchi sheep. Genomic DNA was
extracted from blood of 131 sheep using the salting out method. Bovine and
ovine myostatin gene were used to design PCR primers. SSCP analysis was carried
out to detect discrepancies between animals. Growth traits we included were
birth, weaning, 6 month and yearling weights, estimated by best linear unbiased
tests. The effect of myostatin genotype on sheep EBVs for growth traits were
determined by least squares means GLM. From 11 sets of primers, only primers 3
and 8 which amplify parts of intron 2 of the gene revealed different
Single-Strand Conformation Polymorphism bands. Animals with A3A3 genotype had
higher birth weight compared to A3B3 and B3B3 genotypes (P<0.05). No
significant associations were found between genotypes of My 8 and growth
traits. Results suggest that the polymorphisms may be markers linked to a
specific locus which control growth traits.
Key words: Baluchi sheep,
Myostatin, polymorphism, SSCP.