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OJVRTM
Online Journal of Veterinary
Research ©
Volume 15 (2): 177-185, 2011. Redacted 2018.
Effect of pH and temperature on
growth and fatty acids composition of Mycoplasma
capricolum subsp. capripneumoniae
A.Z.S.A. Zaki1, H. Boehnel2,
S. H. Babiker3, K. M. Suleiman1
1Dept. of Microbiol,
F. of Vet. Med.,
University of Khartoum, Khartoum North, Sudan. 2George-August-University,
Tropentierhygiene, Kellnerweg
6, Goettingen, Germany. 3Central
Veterinary Research Laboratories, Alamarat, Khartoum,
Animal Resources Research corporation, Sudan.
ABSTRACT
Zaki AK, Boehnel H, Babiker SH, Suleiman KM, Effect of pH and temperature on
growth and fatty acids composition
of Mycoplasma capricolum. Capripneumoniae,
Onl J Vet Res, 15 (2): 177-185, 2011. The
growth and cellular long chain fatty acid composition of Mycoplama
capricolum subsp. caprineumopniae
(Mccp), grown in
Hayflick´s medium in a bioreactor at different incubation temperatures
(range: 32– 40°C) and pH values (range: 7.0-8.0), were studied. Gas liquid
chromatographic analysis of different cultures revealed five major peaks which
correspond to palmitoleic, palmitic, linoleic, oleic
and stearic fatty acids. The % concentrations of palmitoleic
acid remained stable at different incubation temperatures, however it increased
at 38°C. Palmitic acid concentration was at high levels at 32 and 34°C then
decreased at 36 and 37°C and increased again at 38°C. The linoleic, oleic and
stearic acids concentration were affected negatively by the increase in temperature.
Palmitoleic acid concentration of the cells remained
almost stable while that of palmitic acid increased gradually to reach a higher
concentration at pH 7.8 and then dropped at pH 8.0. Linoleic, oleic and stearic
acids were at high concentrations at pH 7.4 then decreased gradually with the
increase of the pH. The different incubation
temperatures and pH values were found to have direct variable effects on the
viable count and optical densities of Mycoplasma cultures. At optimal growth
parameters (pH 7.7 and temp. 37°C), palmetic and
linoleic acids showed higher concentration compared to other detected fatty
acids. The results of this study have demonstrated the importance of optimizing
the factors which affect growth of Mccp cells
in order to obtain a homogeneous and stable product with high viable cell
counts that is suitable for vaccine production.
Key words: Mycoplasma capripneumoniae, Bioreactor, Fatty acids, Gas liquid
chromatography.
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