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OJVRTM

Online Journal of Veterinary Research©

(Including Medical and Laboratory Research)

Established 1994
ISSN 1328-925X

 

Volume 27 (10): 583-586, 2023.

Extraction of Mycobacterium avium paratuberculosis DNA from goat intestine.

 

Khalideh Azari1, Mehdi Golchin1, Reza Kheirandish1 and Mojtaba Alimolaei1, 2

 

1Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, 2Razi Vaccine and Serum Research Institute- Kerman branch, Kerman, Iran.

 

ABSTRACT

 

Azari K, Golchin M, Kheirandish R, Alimolaei M., Extraction of Mycobacterium avium paratuberculosis DNA from goat intestine, Onl J Vet Res., 27 (10): 583-586, 2023. We compared kits, conventional phenol-chloroform-isoamyl alcohol-proteinase K and detergent to extract DNA from intestinal tissue of goat with Johne’s disease by Mycobacterium avium subsp. paratuberculosis for DNA quantity and quality at 260/280 nm. We found significant differences between protocols suggesting kits had highest yields and conventional method least whereas conventional method had highest and stool kit least purity.

 

Keywords: Mycobacterium avium, DNA extraction, Novel, Intestine.

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