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Online Journal of Veterinary Research©
(Including Medical and Laboratory Research)
Volume 27 (10): 583-586, 2023.
Extraction of Mycobacterium avium paratuberculosis DNA from goat intestine.
Khalideh Azari1, Mehdi Golchin1, Reza Kheirandish1 and Mojtaba Alimolaei1, 2
1Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, 2Razi Vaccine and Serum Research Institute- Kerman branch, Kerman, Iran.
Azari K, Golchin M, Kheirandish R, Alimolaei M., Extraction of Mycobacterium avium paratuberculosis DNA from goat intestine, Onl J Vet Res., 27 (10): 583-586, 2023. We compared kits, conventional phenol-chloroform-isoamyl alcohol-proteinase K and detergent to extract DNA from intestinal tissue of goat with Johne’s disease by Mycobacterium avium subsp. paratuberculosis for DNA quantity and quality at 260/280 nm. We found significant differences between protocols suggesting kits had highest yields and conventional method least whereas conventional method had highest and stool kit least purity.
Keywords: Mycobacterium avium, DNA extraction, Novel, Intestine.