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Online Journal of Veterinary Research©
(Including Medical and Laboratory Research)
Volume 26 (3): 238-241, 2022.
Culture media for mycobacterium avium subsp. avium from
naturally infected lofts of domestic pigeons.
M Mayahi¹, N Mosavari ², S Esmaeilzadeh ³, K Parvandar Asadollahi¹
1Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz,. Razi Vaccine and 2Serum Research Institute, Karaj, 3Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Iran.
Mayahi M, Mosavari N, Esmaeilzadeh S, Asadollahi KP., Culture media for mycobacterium avium subsp. avium from naturally infected lofts of domestic pigeons, Onl J Vet Res., 26 (3): 238-241, 2022. We report growth of Mycobacterium avium subsp. avium on glycerinated Lowenstein-Jensen medium, pyruvate-enriched Lowenstein-Jensen medium, mycobactin J-supplemented Herrold-egg yolk medium and plain Herrold-egg yolk medium. Eighty pigeons from a group of 600 pigeons were selected for clinical signs and poor health then euthanized, necropsied and followed by bacterial culture on specific media for Mycobacterium avium subsp. avium. Mycobacterium avium subsp. avium were isolated from 51 pigeons. All acid fast basil isolates were tested by PCR assays targeting 16S rRNA, IS1245 and IS901 genes. We found shorter incubation and most colony growth in mycobactin J-supplemented Herrold-egg yolk media. Findings suggest mycobacteria mycobactin as a growth factor.
Key words: Mycobacterium avium subsp. avium, pigeon, culture, PCR, mycobactin.