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OJBTM
Online
Journal of Bioinformatics ©
Volume 13(1):167-183, 2012
In silico
novel MYB transcription factor [EaMYB2R], from Erianthus
arundinaceus.
Pranali Kulkarni1*(MSc)
and Rachayya M. Devarumath1, 2*(PhD)
1Molecular Biology & Genetic Engineering
Division, Vasantdada Sugar
Institute, Manjari (Bk), Tal. Haveli and 2Department of Biotechnology,
University of Pune, Maharashtra, India
ABSTRACT
Kulkarni P, Devarumath RM., In silico novel MYB transcription factor [EaMYB2R from Erianthus arundinaceus.], Online
J Bioinform 13(1):167-183, 2012.
Erianthus arundinaceus
because of its high fibre-yield biomass and
resistance to environmental stresses, is regarded as a
promising biomass crop. Attempts have been made to cross E. arundinaceus with sugarcane to introduce these
character genes into modern cultivars but conclusive results have not been
achieved. The isolation, cloning and In silico
characterization of EaMYB2R gene from E. arundinaceus
encoding a protein belonging to subfamily of R2R3-MYB transcription factor is
herein described. The putative 3D structure of EaMYB2R was generated using
threading assembly refinement [I-TASSER] server. PROCHECK, PROMOTIF and ProSA programs were used to evaluate the stereo chemical
quality of the model and the scores were found within the recommended
intervals. E. arundinaceus showed highest
similarities in R2R3 regions to the MYB transcription factors used for
analysis. The secondary and 3D structures presented through computational modeling studies conferred the structure of DNA binding
sugarcane transcription factor (EaMYB2R)
Key words: MYB Transcription factor, E. arundinaceus,
protein structure modelling, environmental stress,
R2R3 proteins, I-TASSER