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OJBTM
Online Journal of
Bioinformatics ©
Volume 15 (1): 1-34, 2014.
A
novel
strategy of In silico
peptide vaccine design for Streptococcus mutans.
Brittany
Bower
and Anton Dormer MD MS.*
Washington
Adventist University,
7600 Flower Avenue, Takoma Park, Maryland 20912 (301)
891-4229, USA
*Corresponding author
ABSTRACT
Bower
B,
Dormer A., A novel In silico
peptide vaccine design
for Streptococcus mutans, Onl
J Bioinform 15(1):1-34,
2014. Several
attempts have been made towards the
development of a viable vaccine for Streptococcus
mutans (S. Mutans).
This Gram positive bacterium is found within
the oral cavity of humans and is a main inducer of dental
caries. The
objective of this study is to identify
potential peptide vaccine candidates targeted to treat a
primary cause of
dental caries. The hypothesis is that through the use of In silico tools,
T-cell restricted epitopes
targeted to S. Mutans
can be recognized as potentials for therapeutic development. The novel approach
adapted for this
study includes the use of predicted algorithms, QSAR, and
protein fold level
simulation analysis in order to identify B-cell and T-cell
peptide vaccine
candidates from S. Mutans
glucosyltransferase D (GtfD)
protein structure: a key enzyme dental
caries pathogenesis. One
B-cell
(EDDKAASESSQTDAPKTKQA) (20-mer) and five B-cell epitope
originated T-cell
restricted epitopes to MHC Class I HLA-A*0201
(GQDMKITYV)(9-mer), -A*0204
(QIDGKYYYI)(9-mer), -B*2705 (KRYFDDGSG)(9-mer), and MHC
Class II HLA-DRB1*0101
(NNWYYFGSDGVAVTG)(15-mer), and -DRB1*0401
(KIETKISQTQQTQWL)(15-mer) were
identified. The MHC Class I and Class II restricted peptides
predicted in this
study would stimulate T-cytotoxic (CD8+) and T-helper (CD4+)
lymphocytes,
respectively. This design could be used to stimulate CD8+,
CD4+, and B-cells,
all aspects of the adaptive immune system. Therefore, these
restricted epitopes
are designed to induce both a humoral and cellular
immunological responses for
use in a multi-valent vaccine.
As with all epitopes
selected by In silico
tools In Vitro and In Vivo experimental verification will be the
next step.
KEYWORDS:
S.
Mutans, dental caries, vaccine,
enzyme GtfD.