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Online Journal of Veterinary Research©
Volume 23 (7):646-665, 2019.
Pharmaceutics of a low dose progesterone-PMSG nano-liposome
tablet and its effect on vaginal mucus in ewes.
Iman Rasool AlShati and Najlaa S Ibrahim.
Department of Surgery and Obstetrics, College of Veterinary Medicine, University of Baghdad, Iraq
AlShati IR, Ibrahim NS., Pharmaceutics of a low dose progesterone-PMSG nano-liposome tablet and its effect on vaginal mucus in ewes, Onl J Vet Res., 23 (7):646-665, 2019. We describe manufacture and properties of low dose tablets with medroxyprogesterone acetate (P4) nano-liposome muco-adhesive gel and pregnant mare serum gonadotropin (MAG). Liposome was formed from a Bangham thin film of 0.25g phosphatidylcholine and cholesterol entrapped by vortex. The liposome was then hydrated with phosphate buffer and encapsulated with 1000IU pregnant mare serum gonadotropin (PMSG) by vortex. For tablets per se, 0.125g carbopol was dispersed in 6.25 w/w distilled water (2%) to form a gel with pH 4.5. Liposome with P4 or PMSG were mixed with gels 50% 1:2 (w/w). Liposomes retained ~75% P4 and ~69% PMSG with 1-3 and 2-6 lamellae, respectively. P4 liposome measured ~92nm and PMSG ~63nm with osmo-tolerance 0.6-1% NaCl and pH 6-8. The tablets were inserted intra-vaginally in groups of 10 ewes each and compared with standard P4 sponges (MAPS). Compared with non-treated or empty tablet controls or ewes given MAPS, the Spinnbarkeit mucus score, mucus protein, sodium, potassium, calcium and magnesium were higher (P < 0.05) with MAG tablet whereas chloride and magnesium were similar with MAPS or controls. Mucus pH with MAG or MAPS was higher (P < 0.05) compared with controls.
Keywords: Liposome, muco-adhesive, gel, progesterone, PMSG, tablet, ewe.