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OJVRTM
Online Journal of
Veterinary Research©
Volume 23 (7):646-665, 2019.
Pharmaceutics of a low dose progesterone-PMSG nano-liposome
tablet and its effect on
vaginal mucus in ewes.
Iman Rasool
AlShati and Najlaa S
Ibrahim.
Department of Surgery
and Obstetrics, College of Veterinary Medicine, University of Baghdad,
Iraq
ABSTRACT
AlShati IR, Ibrahim NS., Pharmaceutics of a
low dose progesterone-PMSG nano-liposome tablet and
its effect on vaginal mucus in ewes, Onl J Vet Res.,
23 (7):646-665, 2019. We describe manufacture and
properties of low dose tablets with medroxyprogesterone acetate (P4) nano-liposome muco-adhesive gel
and pregnant mare serum gonadotropin (MAG). Liposome was formed from a Bangham thin film of 0.25g phosphatidylcholine and
cholesterol entrapped by vortex. The liposome was then hydrated with phosphate
buffer and encapsulated with 1000IU pregnant mare serum gonadotropin (PMSG) by
vortex. For tablets per se, 0.125g carbopol was dispersed in 6.25 w/w distilled water (2%) to
form a gel with pH 4.5. Liposome with P4 or PMSG were
mixed with gels 50% 1:2 (w/w). Liposomes
retained ~75% P4 and ~69% PMSG with 1-3 and 2-6 lamellae, respectively. P4
liposome measured ~92nm and PMSG ~63nm with osmo-tolerance
0.6-1% NaCl and pH 6-8. The tablets were inserted
intra-vaginally in groups of 10 ewes each and compared with standard P4 sponges
(MAPS). Compared with non-treated or empty tablet controls or ewes given MAPS,
the Spinnbarkeit mucus score, mucus protein, sodium,
potassium, calcium and magnesium were higher (P < 0.05) with MAG tablet
whereas chloride and magnesium were similar with MAPS or controls. Mucus pH
with MAG or MAPS was higher (P < 0.05) compared with controls.
Keywords:
Liposome, muco-adhesive, gel, progesterone, PMSG, tablet, ewe.
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