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OJVRTM

Online Journal of Veterinary Research

Volume 23 (6):544-550, 2019.


Polymorphism in melatonin receptor MTNR1A gene in Does during breeding and non-breeding seasons.

 

Imad Majeed Almeeni and Najlaa Sami Ibrahim.

 

Department of Surgery and Obstetrics, College of Veterinary Medicine, University of Baghdad, Iraq.

 

ABSTRACT

 

Almeeni IM, Ibrahim NS., Polymorphism in melatonin receptor MTNR1A gene in Does during breeding and non-breeding seasons., Onl J Vet Res., 23 (6):544-550, 2019. Melatonin receptor (MTNR1A) gene may affect seasonal reproduction in goats. We describe single nucleotide polymorphism (SNPs) in goat MTNR1A gene exon 2 amplified by PCR during breeding and non-breeding seasons. Jugular blood (5ml) was taken into EDTA from 20 mixed breed non pregnant goats during breeding and non-breeding seasons for DNA. Exon II of MTNR1A gene was amplified with a primer digested by BsaI restriction enzyme to cleave 279 and 577 bp (C allele) and only one 856bp fragment (A allele) to produce genotypes CC for 279 and 577bp, AA for 856bp and CA for 279, 577 and 856bp. Frequencies were 35% for genotype CC, 52.5% for CA and 12.5% for AA. By Hardy-Weinberg allele frequency of C and A were 0.6125 and 0.3875, respectively. Amplified goat melatonin gene receptor 1A (MTNR1A) had 856bp from 4 homozygous CC, heterozygous CA genotype and homozygous AA genotypes. We found 99% sequence similarity with Capra hircus Gene Bank ID: AF419334.1 with 1349 hits and mutations T154C, C190T, G213A. G218A, G238A, G277A,G292C, G352A, T421C, C424T, C577T, C589A, G763A, G797A, C817T with no polymorphism in non-seasonal homozygote CC genotypes at locus 52 melatonin receptor type 1A (MTNR1A) gene.

 

Keywords: MTNR1A gene, polymorphism, goats.


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