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OJVRTM

Online Journal of Veterinary Research

Volume 24 (6):343-350, 2020.


Methicillin resistant Staphylococcus aureus in buffalo raw milk.

 

Zahraa Kadhim Lafi (MSc), Mawlood Abbas Ali Al-Graibawi (PhD).

 

Zoonosis, College of Veterinary Medicine, University of Baghdad, Iraq.

 

ABSTRACT

 

Lafi ZK, Al-Graibawi MAA., Methicillin resistant Staphylococcus aureus in buffalo raw milk. Onl J Vet Res., 24 (6):343-350, 2020. One hundred buffalo raw milk samples from different locations in Iraq, were tested for methicillin resistant Staphylococcus aureus (MRSA) by streaking, blood agar, morphology, gram stain, biochemistry and PCR for blaz and mecA genes. Bacterial resistance and MRSA was determined by disc diffusion. Staphylococcus aureus(S. aureus) was found in 48% milk samples with high resistance to methicillin (~81%), oxacillin (~77%), cefoxitin (~70%), gentamycin (~50%), and vancomycin (~46%). Phenotypic analyses revealed that out of 48 S. aureus, 39 (81%) were MRSA.Genotypes identified by PCR showed that 28 (71.7%) had blaZ and 22 (56.4%) had mecA genes. Results highlight risk of buffalo milk as a source of MRSA to humans.

 

Keywords: buffalo milk, Staphylococcus aureus, MRSA, antimicrobial susceptibility, mecA gene, blaZ gene.


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