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OJVRTM
Online Journal of
Veterinary Research©
Volume 23 (11):1084-1094, 2019
Mitogen induced lymphocyte proliferation
response to acute heat stress and cortisol in buffaloes.
Mukherjee J, Pandita
S, Huozha R, Kumar V, Ashutosh
M.
Dairy Cattle Physiology Division, NDRI, Karnal, Haryana, India.
ABSTRACT
Mukherjee J, Pandita
S, Huozha R, Kumar V, Ashutosh
M., Mitogen induced lymphocyte proliferation response to acute heat stress and
cortisol in buffaloes, Onl J Vet Res., 23
(11):1084-1094, 2019.
Lactating Murrah buffaloes with high ~9.3L/day (n=6) or low milk
output (~6L/day n=6) were tested for mitogen induced lymphocyte stimulation
index (SI) response to acute heat stress and/or cortisol. Blood was taken every
10 days for 30 days and lymphocytes cultured in RPMI 1640 medium with PHA-P for
24h at 37oC in a humidified CO2 incubator (95% air and 5%
CO2) for mitogen test. Blood was also taken before buffaloes
were exposed to 45șC and 55% RH and 3h after. SI was different
(P<0.01) in buffaloes at different levels of production and 2ng/ml reduced SI
(p<0.05) in both groups. However, during heat stress SI did not vary suggesting
that lymphocytes became resistant to effects of cortisol. We find that our mitogen
induced In vitro lymphocyte proliferation test may be used to assess cell
mediated immunity in buffaloes.
Key
Words: Mitogen, lymphocyte, heat stress, cortisol, buffalo.
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