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Online Journal of Veterinary Research©
Volume 23 (11):1084-1094, 2019
Mitogen induced lymphocyte proliferation response to acute heat stress and cortisol in buffaloes.
Mukherjee J, Pandita S, Huozha R, Kumar V, Ashutosh M.
Dairy Cattle Physiology Division, NDRI, Karnal, Haryana, India.
Mukherjee J, Pandita S, Huozha R, Kumar V, Ashutosh M., Mitogen induced lymphocyte proliferation response to acute heat stress and cortisol in buffaloes, Onl J Vet Res., 23 (11):1084-1094, 2019. Lactating Murrah buffaloes with high ~9.3L/day (n=6) or low milk output (~6L/day n=6) were tested for mitogen induced lymphocyte stimulation index (SI) response to acute heat stress and/or cortisol. Blood was taken every 10 days for 30 days and lymphocytes cultured in RPMI 1640 medium with PHA-P for 24h at 37oC in a humidified CO2 incubator (95% air and 5% CO2) for mitogen test. Blood was also taken before buffaloes were exposed to 45șC and 55% RH and 3h after. SI was different (P<0.01) in buffaloes at different levels of production and 2ng/ml reduced SI (p<0.05) in both groups. However, during heat stress SI did not vary suggesting that lymphocytes became resistant to effects of cortisol. We find that our mitogen induced In vitro lymphocyte proliferation test may be used to assess cell mediated immunity in buffaloes.
Key Words: Mitogen, lymphocyte, heat stress, cortisol, buffalo.