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OJVRTM
Online Journal of
Veterinary Research©
Volume 20(3):205-209, 2016.
Aminoglycoside-modifying enzyme
encoding genes in methicillin-resistant staphylococcus
aureus isolated
from
individuals working with sheep routinely fed aminoglycoside antibiotics.
Mohammad
Mostafa Nazari, Farhad Mousakhani.
Department of
Microbiology and Cell Bio-technology, Faculty of Veterinary Medicine, Karaj
Branch, Islamic Azad University, Alborz, Iran
ABSTRACT
Mostafa Nazari M, Mousakhani F., Aminoglycoside-modifying enzyme encoding
genes in methicillin-resistant staphylococcus aureus isolated from individuals
working with sheep routinely fed aminoglycoside antibiotics, Onl J Vet Res., 20(3):205-209, 2016. Aminoglycoside antibiotic
susceptibility patterns of 136 methicillin-resistant staphylococcus aureus (MRSA) positive isolates from 69 individuals
in contact with sheep fed antibiotics were determined and genes encoding the
aminoglycoside-modifying enzymes (AMEs) were analyzed. MRSA were subjected to
multiplex PCR to determine the distribution of staphylococcal cassette
chromosome mec (SCCmec)
types. Resistance rates were gentamicin 71, neomycin 74, tobramycin 78,
kanamycin 81 and amikacin 89%. aac(6 ́)-Ie-aph(2˝) (87%)
was the predominant AME gene and coexistence of three AME genes was detected in
19% of isolates. Of 136 isolates, 127 (93%) were identified as SCCmec type III or IIIA and 9 (7%) categorized as SCCmec type IV. The majority of MRSA isolates belonged to SCCmec III or IIIA and carried the aac(6 ́)-Ie-aph (2˝) gene.
Keywords:
Aminoglycoside-modifying enzymes, Methicillin-resistant Staphylococcus aureus,
live-stock antibiotic resistance.
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