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Online Journal of Veterinary Research©
Volume 24(9): 526-536, 2020.
Effect of methamphetamine on uterine tissue, anxiety and apoptotic genes in female Wistar rats.
Zahra Goudarzi1, Seyed Ebrahim Hoseini1, Davood Mehrabani2,3,4,5, Seyedeh Sara Hashemi3, Amin Derakhshanfar6, Feridoun Karimi-Busheri5
1Department of Biology, Shiraz Branch, Islamic Azad University, Shiraz, 2Stem Cell Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran, 3Burn and Wound Healing Research Center, Shiraz University of Medical Sciences, Shiraz, Iran, 4Center of Comparative and Experimental Medicine, Shiraz University of Medical Sciences, Shiraz, Iran, 5Department of Oncology, University of Alberta, Edmonton, Alberta, Canada, 6Diagnostic Laboratory Sciences and Technology Research Center, School of Paramedical Sciences, Shiraz University of Medical Sciences, Shiraz, Iran *Correspondence: Seyed Ebrahim Hoseini, PhD., Professor, Department of Biology, Shiraz Branch, Islamic Azad University, Shiraz, Iran, Tel: +98-09171183917, email: firstname.lastname@example.org
Goudarzi Z, Hoseini SE, Mehrabani D, Hashemi SS, Derakhshanfar A, Karimi-Busheri F., Effect of methamphetamine on uterine tissue, anxiety and apoptotic genes in female Wistar rats, Onl J Vet Res., 24(9): 526-536, 2020. Methamphetamine use and withdrawal are associated with depression, psychosis, cognitive deficits and sleep disorders. However there is little data for female users. We describe uterine histological, molecular and behavioral changes in uterine tissue following administration in female rats. Three groups of 15 female Wistar rats each were injected subcutaneously every 12 hours 2mg/kg methamphetamine in 0.6mL saline (1), 0.6mL saline (2) and 0.6ml distilled water controls (3) for 14 days. Histological changes in uterine tissue were evaluated at 7, 14 and 21 days and apoptotic Bax and Bcl-2 genes at day 21. Compared with controls, by Y maze tests we found significant (P < 0.01) increase (~1-4 fold) in anxiety in rats given methamphetamine. In di-estrus uterus we observed atrophy of glands and walls, hyperplasia and papillary formation. We found increased expression of apoptotic Bax gene but decreased Bcl-2. Our findings suggest that methamphetamine may induce significant uterine pathology and anxiety in female Wistar rats.
Key words: Methamphetamine, Uterus, Anxiety, Apoptosis, Rat.