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OJVRTM
Online Journal of
Veterinary Research©
Volume 24(9): 526-536, 2020.
Effect of methamphetamine on uterine tissue, anxiety and
apoptotic genes in female Wistar rats.
Zahra Goudarzi1, Seyed Ebrahim Hoseini1, Davood
Mehrabani2,3,4,5, Seyedeh Sara Hashemi3,
Amin Derakhshanfar6, Feridoun
Karimi-Busheri5
1Department of Biology, Shiraz Branch,
Islamic Azad University, Shiraz, 2Stem Cell Technology Research
Center, Shiraz University of Medical Sciences, Shiraz, Iran, 3Burn
and Wound Healing Research Center, Shiraz University of Medical Sciences,
Shiraz, Iran, 4Center of Comparative and Experimental Medicine,
Shiraz University of Medical Sciences, Shiraz, Iran, 5Department of
Oncology, University of Alberta, Edmonton, Alberta, Canada, 6Diagnostic
Laboratory Sciences and Technology Research Center, School of Paramedical
Sciences, Shiraz University of Medical Sciences, Shiraz, Iran *Correspondence:
Seyed Ebrahim Hoseini, PhD., Professor, Department of Biology, Shiraz
Branch, Islamic Azad University, Shiraz, Iran, Tel: +98-09171183917, email:
mehrabad@outlook.com
ABSTRACT
Goudarzi Z, Hoseini
SE, Mehrabani D, Hashemi
SS, Derakhshanfar A, Karimi-Busheri
F., Effect of methamphetamine on uterine tissue, anxiety and apoptotic genes in
female Wistar rats, Onl J
Vet Res., 24(9):
526-536, 2020. Methamphetamine use and withdrawal
are associated with depression, psychosis, cognitive deficits and sleep
disorders. However there is little data for female users. We describe uterine histological,
molecular and behavioral changes in uterine tissue following administration in female
rats. Three groups of 15 female Wistar rats each were
injected subcutaneously every 12 hours 2mg/kg methamphetamine in 0.6mL saline
(1), 0.6mL saline (2) and 0.6ml distilled water controls (3) for 14 days.
Histological changes in uterine tissue were evaluated at 7, 14 and 21 days and apoptotic
Bax and Bcl-2 genes at day 21. Compared with
controls, by Y maze tests we found significant (P < 0.01) increase (~1-4 fold) in anxiety in rats given methamphetamine.
In di-estrus uterus we observed atrophy of glands and walls,
hyperplasia and papillary formation. We found increased expression of apoptotic
Bax gene but decreased Bcl-2. Our findings suggest
that methamphetamine may induce significant uterine pathology and anxiety in female
Wistar rats.
Key words:
Methamphetamine, Uterus, Anxiety, Apoptosis, Rat.