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OJVRTM

Online Journal of Veterinary Research

Volume 25(1): 7-14, 2021.


Methicillin resistant Staphylococcus aureus (mec-A) gene isolated from horses with toxic shock syndrome.

 

Sherein I Abd El-Moez 1, Ahmed FY2, Wagdy KB Khalil3, Omaima H. Ezzo 3

 

1Department of Microbiology and Immunology, 2Department of Animal Reproduction and A.I, 2,3Department of Cell Biology, National Research Center, Giza, Egypt.

ABSTRACT

 

El-Moez SA, Ahmed FY, Khalil W, Ezzo OH., Methicillin resistant Staphylococcus aureus (mec-A) gene isolated from horses with toxic shock syndrome, Onl J Vet Res., 25(1): 7-14, 2021. We report incidence of multiple drug resistant S. aureus (MRSA) in excretions and tissues of infected and dead Arabian and hybrid horses suffering 18.8% mortality rate due to toxic shock syndrome. A QT-PCR for mec-A gene was performed to detect drug resistance in S aureus and isolated 6 different strains. We tested buffalo and cow milk and goat and mare colostrum probiotics on growth of MRSA by disk diffusion. Mec-A gene was found in all samples of MRSA taken from horses. Cow milk probiotics L acidophilus (9.67mm), mare (8.5mm) and goat 8.14mm colostrum exhibited most inhibition of MRSA. Buffalo milk (0mm) and bifido-bacterium (0 -2.6mm) had no useful activity against these bacteria. Findings suggest that probiotic L. acidophilus may be useful in controlling MRSA and dogs should not be in contact with stabled horses.

 

Keywords: MRSA; Quantitative-PCR; mec-A gene; Toxic Shock Syndrome; Equine; Probiotic.


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