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OJVRTM

Online Journal of Veterinary Research©

Volume 24(12): 688-692, 2020.


Methicillin Resistant Staphylococcus aureus isolated from surgical site patients.

 

Sherein I. Abd El-Moez, Sohad M Dorgham, Eman Abd El-Aziz.

 

Department of Microbiology and Immunology, National Research Center, Giza, Egypt.

 

ABSTRACT

 

El-Moez SI, Dorgham SM, El-Aziz EA., Methicillin Resistant Staphylococcus aureus isolated from surgical site patients, Onl J Vet Res., 24(12): 688-692, 2020. We report methicillin resistant Staphylococcus aureus (MRSA) from hospital patientís pus, sputum and blood samples from surgical sites determined by mec-A gene quantitative-PCR. We then tested the isolates against various antibiotics and probiotic lactobacilli from colostrum, milk and fecal samples of mares, cows or goats. Strains were plated onto Mueller Hinton agar plates. We found mec-A gene in 6 of 7 samples. Cefobid and claforan and probiotics B. subtilus, L. acidophilus from colostrum of mares and L. acidophilus from goat colostrum were most effective against MRSA. However, L. acidophilus from Buffalo-cow milk showed no activity. We find that mec-A gene was involved in all MRSApatients with severe skin infections.

 

Keywords: MRSA; Quantitative-PCR; mec-A gene; Antibiotic sensitivity; Human; Probiotics.


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