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OJVRTM
Online Journal of Veterinary Research©
(Including
Medical and Laboratory Research)
Established
1994
ISSN
1328-925X
Volume 28(3): 146-155, 2024.
Antibiotic probiotic resistance by Staphylococcus
aureus isolated from surgical site patients.
Sherein I Abd
El-Moez, Sohad M Dorgham, Eman Abd
El-Aziz.
Department of Microbiology and Immunology, National Research Center, Giza,
Egypt.
ABSTRACT
El-Moez SI, Dorgham SM, El-Aziz EA., Antibiotic probiotic resistance by Staphylococcus aureus isolated from
surgical site patients, Onl J Vet
Res., 28(3): 146-155, 2024. We report methicillin
resistant Staphylococcus aureus (MRSA) from hospital patient’s pus,
sputum and blood samples from surgical sites determined by mec-A gene quantitative-PCR. We then tested the
isolates against various antibiotics and probiotic lactobacilli from colostrum,
milk and fecal samples of mares, cows or goats. Strains were plated onto
Mueller Hinton agar plates. We found mec-A gene in 6 of 7 samples. Cefobid and claforan and
probiotics B. subtilus, L.
acidophilus from colostrum of mares and L. acidophilus from
goat colostrum were most effective against MRSA. However, L.
acidophilus from Buffalo-cow milk showed no activity. We find
that mec-A gene was involved in
all MRSA patients with severe skin infections.
Keywords: MRSA;
Quantitative-PCR; mec-A gene; Antibiotic
sensitivity; Human; Probiotics.
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