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Online Journal of Veterinary Research

Volume 25 (1): 70-78, 2021.

Effect of s-adenosyl methionine on serum progesterone, estrogen and ovarian follicles

in albino rats exposed to carbon tetrachloride.


Jalal A Al-Saedi1 and Jawad K Arrack2


1Al-Farahidi University Medical laboratories, Baghdad, 2Department of Physiology and Pharmacology, College of Veterinary Medicine, University of Baghdad, Iraq.




Al-Saedi JA, Arrack JK., Effect of s-adenosyl methionine on serum progesterone, estrogen and ovarian follicles in albino rats exposed to carbon tetrachloride, Onl J Vet Res., 25 (1): 70-78, 2021. We describe effect of a Schiff base derivative of methionine (s-adenosyl methione) on ovarian function and structure in albino female rats exposed to carbon tetrachloride. Groups of 10 rats each were injected intraperitoneally 48mg/kg s-adenosyl methionine, [0.1ml] 100mg/kg carbon tetrachloride or both in 0.5mg/kg olive oil twice a week for six weeks. Controls were injected 0.5ml DMSO. Blood was taken at days 0, 21 and 42. Compared with controls, in rats given only methionine, serum progesterone increased ~30% at day 21 and 42% by day 42 whereas in those injected carbon tetrachloride, declined ~39 and 48% (P < 0.05). In rats given both, there were no significant changes. Serum estrogen increased ~20% by day 42 in rats given methionine but declined 41 and 51% by day 42 in those given carbon tetrachloride. However, in rodents injected both we found no significant variations. Microscopy revealed normal ovarian structure and follicles in control rats given DMSO or methionine with increased number of normal follicles. However, in those given only carbon tetrachloride we found degeneration of mature follicles, extensive congestion and severely degenerated trophoblastic cell. In rats given carbon tetrachloride together with s-adenosyl we observed normal histology suggesting that the latter may ameliorate toxicity induced by the toxin.

Keywords: Schiff base, methionine, Carbone tetrachloride, Antioxidant.