MAIN


1996-2018. All Rights Reserved. Online Journal of Veterinary Research. You may not store these pages in any form except for your own personal use. All other usage or distribution is illegal under international copyright treaties. Permission to use any of these pages in any other way besides the before mentioned must be gained in writing  from the publisher. This article is exclusively copyrighted in its entirety to OJVR. This article may be copied once but may not be, reproduced or re-transmitted without the express permission of the editors. This journal satisfies the refereeing requirements (DEST) for the Higher Education Research Data Collection (Australia). Linking: To link to this page or any pages linking to this page you must link directly to this page only here rather than put up your own page


 

OJVRTM

 

Online Journal of Veterinary Research

 

Volume 21(11):720-727, 2017...


Metachromasia of exfoliated vaginal epithelial cells as marker of ovarian activity in ewes

 

Nazih Wayes Zaid.

 

Veterinary Obstetrics PhD, Department of Surgery and Obstetrics, College of Veterinary Medicine, University of Baghdad, Iraq E-mail: nazih_keplan@yahoo.com.

 

ABSTRACT

 

Nazih Wayes Zaid., Metachromasia of exfoliated vaginal epithelial cells as marker of ovarian activity in ewes, Onl J Vet Res., 21(11):720-727, 2017. Five pre-pubertal, 5 pubertal and 15 older Awassi ewes were swabbed for exfoliated vaginal epithelial cells. Swabs and blood were taken every 15 days. Swabs were stained with methylene blue. Cells from ewes in estrus or pregnant, shifted blue to red-blue or purple, with status confirmed by ultrasound and microscopy. At estrus, progesterone varied 1.9-19.1 ng/ml and estrogen 52.4-88.0 pg/ml and in pregnancy, 6.5-25.1 and 10.1-99.5 pg/ml. Cells taken postpartus, showed no change at 2 weeks. Inactive ovaries were confirmed by sonar, 0.6 ng/ml progesterone and 4.1 pg/ml estrogen. Cells swabbed in late postpartum from 11 ewes with cyclic activity shifted light to dark blue with 2.2 ng/ml progesterone and 5.7 pg/ml estrogen. Uterine infections did not affect color. Results suggested that color of exfoliated vaginal cells may be a useful marker of ovarian cyclical activity in ewes. At pre-puberty, no color change in inactive ovaries was found, supporting the findings.

Keywords: Metachromasia, ewes, vaginal, epithelium, ovarian, sex hormones.


MAIN

 

FULL-TEXT(SUBSCRIPTION) OR PURCHASE ARTICLE