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Online Journal of Veterinary Research©
Volume 23 (9):852-872, 2019.
Plasma metabolomics in mice gavaged aspirin with or without liposome during luteal and follicular phases.
Shrooq J Jassim and Mohanad A Al-Bayati.
Department of Radiation, Radiotherapy, Medical Technical Institute, Middle Technical University, Baghdad and Department of Physiology and Pharmacology, College of Veterinary Medicine, University of Baghdad, Baghdad-Iraq
Jassim SJ, Al-Bayati MA., Metabolomics in plasma of virgin female mice gavaged aspirin with or without liposomes during luteal and follicular phases., Onl J Vet Res., 23 (9):852-872, 2019. Metabolomics assesses metabolites, small molecule intermediates and products of metabolism. We report metabolomics in plasma of mice given liposome aspirin during ovarian luteal and follicular phases. Groups of 5 virgin female Albino mice were each gavaged 1.4 or 0.7mg/kg aspirin with or without liposome daily for 25 days. Controls were not treated. Metabolites in plasma were extracted in methanol-chloroform-water and identified by gas chromatography mass spectrophotometry. In mice during luteal phase given aspirin with or without liposome we detected 47 metabolites consisting of amino acids (14%), carbohydrates (43%), fatty acids (36%) and aspirin metabolites (7%). Compared with controls (P < 0.05), 1.4 mg/kg aspirin or liposomal aspirin reduced plasma propanoic, palmitic, glycine, alanine and eicosanoic acid but had no effect on glucose. At only 0.7mg/kg aspirin also reduced (P < 0.05) same compounds but less. We find that plasma Indol-3-acetic acid and urea may be suitable biomarkers of aspirin ovarian toxicity.
Key words: metabolomics, aspirin, liposome mice.