1994-2019. All Rights Reserved. Online Journal of Veterinary Research. You may not store these pages in any form except for your own personal use. All other usage or distribution is illegal under international copyright treaties. Permission to use any of these pages in any other way besides the before mentioned must be gained in writing from the publisher. This article is exclusively copyrighted in its entirety to OJVR publications. This article may be copied once but may not be, reproduced or re-transmitted without the express permission of the editors.


 Online Journal of Veterinary Research

Volume 22 (7):596-601, 2018.

Effect of gentamycin and calcium EDTA on mesenteric lymph nodes in rabbits

following oral Salmonella typhimurium infection.


Ahmed Sami Jarad1, Zaid Yaseen Ibrahim1, Rafid, K. Ali1, Mustafa Salah Hasan1, Maher Saber Owain2, Mohammed Ali Hussein1


1Department(s) of Internal and Preventive Veterinary Medicine, College(s) of Veterinary Medicine, University of Fallujah, 2University of Tikrit, Iraq.




Jarad AS, Ibrahim ZY, Ali RK, Hasan MS, Owain MS, Hussein MA., Effect of gentamycin and calcium EDTA on mesenteric lymph node of rabbits following Salmonella typhimurium infection, Onl J Vet Res., 22 (7):596-601, 2018. Five groups of 5 mixed breed rabbits each were gavaged 5X109 CFU Salmonella typhimurium with or without 0.05ml/kg gentamicin (5mg/ml) and/or 40mg/kg Ca-EDTA 40mg/kg, 24h post infection. Controls (n=5) were neither challenged nor treated. Necropsies were performed at 48 and 96hrs after appearance of signs. At necropsy, compared with controls, in those challenged with S. typhimurium and/or treated, nodes appeared edematous, soft, hemorrhagic, and enlarged. In rabbits challenged with S. typhimurium only, there was severe lymphocyte depletion and necrosis. In those challenged but given gentamicin only, or EDTA only, there was still lymphocytosis and macrophage infiltration with defined zones of necrosis, Finally in rabbits challenged with S typhimurium, but given both Gentamycin and EDTA, lymph nodes appeared normal macroscopically and microscopically. We find that a single dose of gentamycin with EDTA given 24h after challenge, may prevent mesenteric lymph node pathology induced by S typhymurium in rabbits


Key words: Salmonella, rabbits, Mesenteric lymph nodes, Ca-EDTA, gentamicin.