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OJVR
Online Journal of Veterinary Research©
(Including Medical and Laboratory Research)
Established 1994
ISSN 1328-925X
Volume 25 (7):487-494, 2021.
Staphylococcus aureus producing biofilm and mastitis in lactating does.
1Ali Hussein AL-Dujaily, 2Alaa Kamil
Mahmood
1Department of Veterinary Clinical Sciences,
Faculty of Veterinary Medicine, University of Kufa, 2Department
of Internal and Preventive Veterinary Medicine, Faculty of Veterinary Medicine,
University of Baghdad, Iraq.
ABSTRACT
AL-Dujaily AH,
Mahmood AK., Staphylococcus
aureus producing biofilm and mastitis in lactating does, Onl J Vet Res., 25 (7):487-494, 2021. We
report mastitis induced by biofilm generating Staphylococcus aureus in lactating does. Lactating does
(n=25) were tested for lack of mastitis by palpation, California mastitis (CMT)
and somatic cell (SCC) tests. We collected milk from does with
mastitis for biofilm production
by S. aureus detected as black
colonies on Congo red agar. Healthy does were then inoculated 1 mL PBS with 1.5
× 108 CFU biofilm
producing S. aureus through teat canal. By CMT, we found sub-clinical mastitis
in ~30% and ~44% severe cases. Goats exhibited signs of mastitis 24h after inoculation.
Compared with controls, temperature increased 4.8%, heart
beats 16% and respiratory rate 35%. Severe mastitis yielded high CMT scores (3+)
by 48 h with a 6.9 fold increase in SCC. By 48-72h infected udder halves felt
warm, swollen, painful, hyperemic and milk output fell
and milk samples clotted. We find that biofilm-producing S. aureus could
be extremely pathogenic for lactating does.
Key words: Staphylococcus aureus, mastitis, lactating local breed
goats.