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Online Journal of Veterinary Research

(Including Medical and Laboratory Research)

Established 1994
ISSN 1328-925X


 Volume 25 (7):487-494, 2021.

Staphylococcus aureus producing biofilm and mastitis in lactating does.


1Ali Hussein AL-Dujaily, 2Alaa Kamil Mahmood


1Department of Veterinary Clinical Sciences, Faculty of Veterinary Medicine, University of Kufa, 2Department of Internal and Preventive Veterinary Medicine, Faculty of Veterinary Medicine, University of Baghdad, Iraq.




AL-Dujaily AH, Mahmood AK., Staphylococcus aureus producing biofilm and mastitis in lactating does, Onl J Vet Res., 25 (7):487-494, 2021. We report mastitis induced by biofilm generating Staphylococcus aureus in lactating does. Lactating does (n=25) were tested for lack of mastitis by palpation, California mastitis (CMT) and somatic cell (SCC) tests. We collected milk from does with mastitis for biofilm production by S. aureus detected as black colonies on Congo red agar. Healthy does were then inoculated 1 mL PBS with 1.5 108 CFU biofilm producing S. aureus through teat canal. By CMT, we found sub-clinical mastitis in ~30% and ~44% severe cases. Goats exhibited signs of mastitis 24h after inoculation. Compared with controls, temperature increased 4.8%, heart beats 16% and respiratory rate 35%. Severe mastitis yielded high CMT scores (3+) by 48 h with a 6.9 fold increase in SCC. By 48-72h infected udder halves felt warm, swollen, painful, hyperemic and milk output fell and milk samples clotted. We find that biofilm-producing S. aureus could be extremely pathogenic for lactating does.


Key words: Staphylococcus aureus, mastitis, lactating local breed goats.