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Online Journal of Veterinary Research©
Volume 22 (3):174-178, 2018.
Effects of Pistacia atlantica supplement on expression of immune response genes in cecum of Ross 308 broilers.
Shahraki E, MS.
Research Center of Special Domestic Animal, University of Zabol, Zabol, Iran
Shahraki E, MS., Effects of Pistacia atlantica supplement on expression of immune response genes in cecum of Ross 308 broilers, Onl J Vet Res., 22 (3): 174-178, 2018. One-day-old Ross 308 male broiler chicks were fed a basal diet supplemented with 0.5% Pistacia atlantica (mastic gum) powder for 28 days. Controls were fed only the basal diet. On day 28, cecum samples were excised (3 per treatment) to evaluate changes in expression of genes TLR2, TLR4, MyD88, NF-kB, IL-6, IL-10, IL-1 and β-actin by realtime PCR. Compared with controls MyD88 (+1.33), NF-kB (+1.25), and IL-6 (+2.28) increased (P<0.05) in cecum tissue of chicks supplemented P. atlantica. There were no changes in gene expression in controls. However, in broilers fed the supplement we found a decrease (P<0.05) in TLR2 (-0.41), TLR4 (-0.67), IL-10 (-0. 73), and IL-1beta (-0.50) compared with controls. Transcripts of IL-6 (+2.28) and TL-10 (-0.73) had the highest difference compared with control values (P<0.05). The findings suggest that Pistacia atlantica mastic gum supplementation may affect cecum gene expression in broilers.
Keywords: Broiler chicken; Gene expression; Immune response; Mastic gum.