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OJVRTM
Online
Journal of Veterinary Research©
Volume 22 (3):174-178, 2018.
Effects
of Pistacia atlantica
supplement on expression of immune response
genes in cecum of Ross 308 broilers.
Shahraki E, MS.
Research Center of Special
Domestic Animal, University of Zabol, Zabol, Iran
ABSTRACT
Shahraki E, MS., Effects of Pistacia
atlantica supplement on expression of immune response
genes in cecum of Ross 308 broilers, Onl J Vet Res., 22 (3): 174-178, 2018. One-day-old
Ross 308 male broiler chicks were fed a basal diet supplemented with 0.5% Pistacia atlantica (mastic gum) powder for 28
days. Controls were fed only the
basal diet. On day 28, cecum samples were excised (3 per treatment) to evaluate
changes in expression of genes TLR2, TLR4, MyD88, NF-kB, IL-6, IL-10, IL-1 and β-actin by realtime PCR. Compared
with controls MyD88 (+1.33),
NF-kB (+1.25), and IL-6 (+2.28) increased (P<0.05) in
cecum tissue of chicks supplemented P. atlantica. There were no changes in gene expression in
controls. However, in broilers fed the supplement we found a decrease (P<0.05) in
TLR2 (-0.41), TLR4 (-0.67), IL-10 (-0. 73), and IL-1beta (-0.50) compared with
controls. Transcripts of IL-6 (+2.28) and TL-10 (-0.73) had the highest
difference compared with control values (P<0.05). The
findings suggest that Pistacia atlantica mastic gum supplementation may
affect cecum gene expression in broilers.
Keywords: Broiler chicken; Gene expression; Immune response; Mastic gum.
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