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OJVRTM
Online Journal of Veterinary Research©
(Including
Medical and Laboratory Research)
Established
1994
ISSN
1328-925X
Volume 22
(10):994-1002, 2018.
Pasteurella multocida
serotypes A, B and E isolated from Marsh buffalo.
Ibrahim Abbas Mohammed1, Jenan Mahmood Khalaf1, Abdulkarim
Jafar Karim*
1Department of Internal and Preventive Medicine,
College of Veterinary Medicine / University of Baghdad, Baghdad – Iraq.
ABSTRACT
Mohammed IA, Khalaf
JM, Karim AJ., Pasteurella multocida serotypes A, B and E isolated from Marsh
buffalo., Onl J Vet Res., 22 (10):994-1002, 2018. Capsular Serotypes B and E
of P. multocida can cause severe acute hemorrhagic septicemia
(HS) in bovines. Authors describe pasteurella serogroups isolated from buffalo located
in Thi-Qar, Basra and Misan
marshes, Iraq between May 2017 and April 2018. Nasal swabs and blood were collected from 393 buffalo cultured on
enriched selective media for identification of P. multocida
by biochemical, gram stain and commercial API 20E system. Conventional PCR
confirmed findings, genotypes and virulent genes. We determined that 194
buffalo appeared clinically diseased with HS and 199 were normal. Pasteurella multocida
in diseased buffalo was 34% higher compared (P < 0.05) with 14% in healthy subjects. Prevalence in buffalo located at marshes was 25.4% (100 out 393) from nasal swabs but only 1.03% in blood (2 out of 194). Records show that 35% serotypes were A, 63% type B and
only 2% type E all significantly different (P < 0.05). PCR confirmed P. multocida yielding species-specific 460 bp with KMT1T7 and KMT1SP6 primers and reference serotype
A, B and E with characteristic DNA laddering at 1,044 bp,
760 bp, and 511 bp,
respectively. We find that P. multocida, genotypes A, B and E are endemic in diseased and healthy buffaloes
located in marshes in Southern Iraq.
Key words; Pasteurella multocida, type E, Buffaloes,
Iraq.
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