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Online Journal of Veterinary Research©
(Including Medical and Laboratory Research)
Volume 22 (10):994-1002, 2018.
Pasteurella multocida serotypes A, B and E isolated from Marsh buffalo.
Ibrahim Abbas Mohammed1, Jenan Mahmood Khalaf1, Abdulkarim Jafar Karim*
1Department of Internal and Preventive Medicine, College of Veterinary Medicine / University of Baghdad, Baghdad – Iraq.
Mohammed IA, Khalaf JM, Karim AJ., Pasteurella multocida serotypes A, B and E isolated from Marsh buffalo., Onl J Vet Res., 22 (10):994-1002, 2018. Capsular Serotypes B and E of P. multocida can cause severe acute hemorrhagic septicemia (HS) in bovines. Authors describe pasteurella serogroups isolated from buffalo located in Thi-Qar, Basra and Misan marshes, Iraq between May 2017 and April 2018. Nasal swabs and blood were collected from 393 buffalo cultured on enriched selective media for identification of P. multocida by biochemical, gram stain and commercial API 20E system. Conventional PCR confirmed findings, genotypes and virulent genes. We determined that 194 buffalo appeared clinically diseased with HS and 199 were normal. Pasteurella multocida in diseased buffalo was 34% higher compared (P < 0.05) with 14% in healthy subjects. Prevalence in buffalo located at marshes was 25.4% (100 out 393) from nasal swabs but only 1.03% in blood (2 out of 194). Records show that 35% serotypes were A, 63% type B and only 2% type E all significantly different (P < 0.05). PCR confirmed P. multocida yielding species-specific 460 bp with KMT1T7 and KMT1SP6 primers and reference serotype A, B and E with characteristic DNA laddering at 1,044 bp, 760 bp, and 511 bp, respectively. We find that P. multocida, genotypes A, B and E are endemic in diseased and healthy buffaloes located in marshes in Southern Iraq.
Key words; Pasteurella multocida, type E, Buffaloes, Iraq.