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OJVRTM
Online Journal of
Veterinary Research©
Volume 5:54-61, 2001. Redacted 2018.
Diagnosis of Marek’s
Disease Virus (MDV) in commercial chickens by Slot-Blot Hybridization using PCR
based MDV-1 antigen A Gene probe.
Vathsala M1*, Kumanan
K2, Saravanabhava K3
and Gunaseelan L4
Veterinary Assistant Surgeon, Veterinary Dispensary, Soolagiri, Dharmapuri District,
Tamil Nadu (2), Department of Animal Biotechnology, Department of Microbiology,
Madras Veterinary College, TANUVAS, Chennai – 600 007, (3,4) Technical officer,
Tamil Nadu University of Veterinary and Animal Sciences, Madhavaram,
Chennai-600 053, INDIA*Corresponding author
SUMMARY
Vathsala M, Kumanan
K, Saravanabhava K, Gunaseelan
L., Diagnosis of Marek’s Disease Virus
(MDV) in commercial chickens by Slot-Blot Hybridization using PCR based MDV-1
antigen A Gene probe Onl J Vet Res., 5:54-61,
2001. PCR and PCR-based Slot-blot hybridization
were used to diagnose marek’s disease virus (MDV)in commercial chickens. The primers used were specific to
pathogenic MDV-serotype 1. PCR was standardized for field samples with DNA from
diseased birds. Fifty samples were collected from diseased farms and screened
by PCR based probed slot-blot hybridization technique. Primers were prepared
according to the nucleotide sequences of MDV-1 antigen A gene amplified MDV-1
DNA. Thirty samples were found to be positive. PCR was more sensitive in
detecting MDV –1 sequences and more number of samples were screened by
slot-blot hybridization at a single stretch.
KEY WORDS: MDV-1- Marek’s disease -
Pathogenicity gene – PCR - Slot-blot hybridization.