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Online Journal of Veterinary Research©
Volume 23 (3):190-199, 2019.
Genetic diversity and DNA profiling of maize varieties by ISSR-PCR.
Abdulmajed Abdulazez Hammadi al-Rawi, Mutaz Abduljabbar Farhan al-Fahdawi.
Al-Anbar University, College of Pure Science, Department of Biology, Al-Anbar, Iraq.
Hammadi al-Rawi AA, Farhan al-Fahdawi MA., Genetic diversity and DNA profiling of maize varieties by ISSR-PCR, Onl J Vet Res., 23 (3):190-199, 2019. Maize (Zea mays L.) saccharata (Z1), indurate (Z2), indentata (Z3), everta (Z4), tunicate (Z5) and Z. mays amylacea (Z6) cultivars used in Iraq were subjected to 10 ISSR DNA primers for genetic variation, relationship and dendogram fingerprint. We found 65-90 micrograms DNA for each gram of leaf by CTAB with purity range between 1.5-1.85 by PCR and electrophoresis. ISSR of 10 primers amplified 366 and 198bp monomorphic 54.09% and 168bp were polymorphic (45.90%). Our dendogram included one group of Z1, Z2 and another minor Z5, Z4 and Z6 with Z3. Closest genetically by ISSR markers were Z6 and Z3 and most remote Z1 and Z6. The results of the ISSR reactions showed that in all 366 bands, the primer (IS-11) (62) had highest attachment. Genetic distance amongst the six varieties ranged 0.09194 - 0.32896.
Key words: genetic diversity , Zea mays L. , ISSR.