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OJVRTM

Online Journal of Veterinary Research©

Volume 20(12):755-760, 2016.


Connective tissue and lymphocyte density in mesenteric lymph node of cats infected

naturally with feline leukemia virus (FeLV) and FeLV negative controls.

 

Flávia Azevedo Cavalcanti de Melo1, André Rodrigues da Cunha Barreto Vianna1, Anna Clara dos Santos1,

Danila Barreiro Campos2, Kaique Nogueira1, Eduardo Maurício Mendes de Lima1

 

1University of Brasilia, Faculty of Agronomy and Veterinary Medicine, Laboratory of Anatomy of Domestic Animals, 70910-970, Brasília, Distrito Federal, Brazil. 2Federal University of Paraíba Corresponding author: limaemm@unb.br

 

ABSTRACT

 

Melo FAC, Barreto Vianna ARC, Santos AC, Campos DB, Nogueira K, Lima EMM., Density of connective tissue and lymphocytes in mesenteric lymph node of cats infected naturally with feline leukemia virus (FeLV) and FeLV negative controls, Onl J Vet Res., 20(12):755-760, 2016. Feline Leukaemia Virus (FeLV) is a gama-retrovirus which infects domestic cats and wild felines inducing immunosuppression, anemia and lymphoma. Density of connective tissue and lymphocytes in mesenteric lymph node in cats infected naturally by FeLV and negative controls is described. Lymph nodes were excised after euthanasia, cut, fixed and stained to calculate area occupied by lymphocytes and density of connective tissue with image Pro-Plus 6.0®. Mean density of lymphocytes in cats (n=4) with FeLV was 20.45% ± 0.97 and connective tissue density 4.7% ± 0.28. The same values in negative FeLV controls (n=5) were 14.75% ± 0.43 (P < 0.0001) and 3.98% ± 0.18 (P < 0.0383), respectively. An increase in density of lymphocytes in FeLV-infected animals was an unexpected result since the literature describes immunosuppression. The increase suggested  immune reactivity with hyperplasia. 

 

Key-words:  FeLV, immunosuppression, morphometry, lymphocytes, connective tissue, lymphoid tissue, feline.


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