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OJVRTM

Online Journal of Veterinary Research

Established 1994

ISSN 1328-925X

 

Volume 22 (10):914-918, 2018.


Identification of Haemonchus longistipes from camels (Camelus dromedaries) by PCR.

 

Ali I Fadhil, Mohammed TH, Salih Al-Zubaidi

 

Department of Parasitology, Faculty of Veterinary Medicine, University of Baghdad , Iraq.

 

ABSTRACT

 

Ali I Fadhil, Mohammed TH, Salih Al-Zubaidi., Identification of Haemonchus longistipes in camels (Camelus dromedaries) by PCR, Onl J Vet Res., 22 (10):914-918, 2018. Haemonchus spp. "Barber Pole Worm" of Trichostrongyloidea in ruminants particularly in tropical and subtropical areas causes anemia, emaciation and decreased milk production. We report identification for haemonchus spp. in camels (Camelus dromedaries) by PCR. Fifty abomasum samples were taken from slaughtered camels in Iraq from January to September 2018. Gene extraction was done from samples and ITS2 ribosomal DNA was used as a gene marker for PCR for DNA amplification, 15 PCR products were sent to Sangrt sequencer, NICEM, South Korea for DNA sequencing and results confirmed Haemonchus longistipes.

 

Key word : Identification, Haemonchus spp., Camels, PCR, Iraq.


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