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OJVRTM
Online Journal of
Veterinary Research©
Volume
21(6):335-344, 2017.
Keratinase producing bacteria isolated from litter for digestion of
keratin in feathers.
Somayeh Rahimnahal1. Amir
Meimandipour2.
Jamal
Fayazi1*. Ali Asghar Karkhane2.
Mehdi Shamsara2. Mohammad Taghi
Beigi Nassiri1
Department(s) 1Animal Science, Ramin Agriculture and Natural Resources University of
Khuzestan, Mollasani, 2Animal Biotechnology,
National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran,
Iran
ABSTRACT
Rahimnahal S, Meimandipour
A, Fayazi J, Karkhane AA, Shamsara M, Beigi Nassiri MT., Keratinase producing bacteria isolated from
litter for digestion of feather keratin., Onl J Vet
Res., 21(6):335-344,
2017. Keratin
constitutes ~90% broiler feather weight which could be recycled into feed by
keratinase producing microorganisms. Broiler litter samples were cultured in
skim milk and keratine agar for keratinolytic
microbes and isolated by microscope, biochemical characterization and molecular
analysis. Keratin in feathers was extracted for feather meal broth spread on
skim milk agar. Colonies which gave clear zones formed by hydrolysis of skim
milk were re-cultured several times on fresh LB agar medium to produce purified
single colonies. Of 25 strains isolated on skim
milk agar Staphylococcus, Enterococcus, Bacillus safensis, B. Paenibacillus
and Brevibacterium exhibited keranolytic activity. By 16S
rRNA typing, phylogenetic analysis and diameter on clear zone on keratin agar medium, Staphylococcus
lentus SLKr1 and Bacillus safensis BSKr1 exhibited highest keratinolytic activity, high caseinase
activity but were negative to amylase, lecithinase, cellulase and gelatinase. Bacillus safensis BSKr1 and Bacillus safensis BSKr2 also exhibited amylase, cellulase, caseinase and lecithinase activities. Results suggest that Staphylococcus
lentus SLKr1 and Bacillus safensis BSKr1 possess high keratinolytic
activities with potential use for digestion of feather keratin.
Key words: Feathers, Keratin, Keratinase. Broiler litter
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