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Online Journal of Veterinary Research©
Volume 22 (2): 104-114, 2018.
Molecular characterization and antimicrobial susceptibility of Listeria monocytogenes isolated from human placentas and non-human samples.
Gany Kszaal Atshan (MSc), Mawlood Abbas Al-Graibawi (PhD).
Zoonotic Diseases Unit, College of Veterinary Medicine, University of Baghdad, Iraq
Atshan GK, Al-Graibawi MA., Molecular characterization and antimicrobial susceptibility of Listeria monocytogenes isolated from human placentas and non-human samples, Onl J Vet Res., 22 (2): 104-114, 2018. Fifty human placentas and 150 non-human samples from Thi Qar, Iraq, were tested for Listeria monocytogenes by inoculation in broth cultured on Listeria selective Chromogenic and PALCAM agar. Isolates were confirmed by colony morphology, gram stain, biochemistry and PCR’s to detect virulence associated genes iap, inlA and hlyA. A disc diffusion test was performed to detect antimicrobial resistance. L. monocytogenes was found in 7 (8.5%) placentas and 10 (6.6%) non-human samples. PCR revealed that all isolates possessed the iap gene and 15 both inlA and hlyA genes. Human isolates exhibited high resistance to ceftazidime and penicillin G (100%) and chloramphenicol (~86), but high sensitivity to tetracycline, streptomycin, trimethoprim and norfloxacin (100%). Non-human isolates exhibited high resistance to ceftazidime (~90%), penicillin G (~80%) and chloramphenicol (~70%), but high sensitivity to norfloxacin, streptomycin, tetracycline and trimethoprim (100%). Results suggest differences in molecular and antimicrobial susceptibility between L. monocytogenes isolated from human and non-human samples.
Keywords: Listeria monocytogenes, Molecular characterization, iap gene, inlA gene, hly genes, PCR, Listeriosis.