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OJVRTM
Online Journal of
Veterinary Research©
Volume 23 (7):628-645, 2019.
Evaluation of a muco-adhesive nano liposome low dose progesterone-PMSG
intra-vaginal
tablet on estrus, ovulation and lambing in ewes.
Iman Rasool Al-Shati and Najlaa Sami Ibrahim.
1Department
of Surgery and Obstetric; Faculty of College of Veterinary Medicine University
of Baghdad, Iraq.
SUMMARY
Al-Shati IR,
Ibrahim NS., Evaluation of a muco-adhesive nano liposome low dose progesterone-PMSG intra-vaginal
tablet on estrus, ovulation and lambing in ewes, Onl J
Vet Res., 23 (7):628-645, 2019. We evaluated an
intravaginal adhesive muco-nano liposomes tablet with
low dose 5mg progesterone (P4) and 50IU pregnant mare serum gonadotropin PMSG (MAG) on onset and duration
of estrus, ovulation, pregnancy,
lambing, litter, twins and serum P4 in groups on 10 ewes each. The formulations
were retained in a 50% hydrogel 0.5cm radius 0.1cm thick tablet placed on the
dorsal wall of the vagina. Results were compared with standard higher dose 50mg
P4 and 200IU PMSG intra-vaginal sponges (MAPS) in 10 ewes. Ten control ewes
were inserted empty tablet and another 10 were not treated. Onset, duration of estrus, ovulation were
recorded and synchronization and pregnancy confirmed by blood P4 and
ultrasound. Liposomes retained ~81% P4 and ~88% PMSG. Mean induction of estrus
was 90% with MAG and 80% with MAPS compared with only 40-50% in controls (P
< 0.05). In ewes inserted MAG, mean estrus was evident at ~34h, MAPS ~55h
and controls 71-82h (P < 0.05) which then lasted 37h, 29h and 18-19h, respectively (P < 0.05). Mean pregnancy and lambing with MAG or MAPs was
90% and controls 40-50% (P < 0.05). Mean litter size was 1.33
with MAG, 1.25 with MAPS, and 1 in controls. At day 17 after insertion, serum progesterone
reached 4.87±0.15ng/ml in ewes inserted MAPS and 2.93±0.99ng/ml in ewes
inserted MAGS but only 1.02±0.01 ng/ml in controls inserted empty tablet and
1.64±0.03 ng/ml in those not treated. Progesterone levels in those give MAPS
were higher than MAG. Lambing and twinning were correlated with higher
progesterone. We found no difference in control lamb genders but more females
(~66%) in ewes inserted MAG or MAPS. Birth weight in MAPS ranged 3.73-4.41kg MAG
3.47-4.01 and controls 4.40-4.72kg. We find that the low dose P4 and PMSG
tablet yielded similar or better reproductive outcomes in ewes compared with
standard high dose sponges.
Key words:
liposome, PMSG, ewe, MAG Lipo P4, Lipo
PMSG, Iraq, Estrous synchronization, superovulation. Raw data Provided.
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