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OJVRTM
Online
Journal of Veterinary Research©
(Including Medical
and Laboratory Research)
Established 1994
ISSN 1328-925X
Volume 28(7): 423-433,
2024.
Effect of cobalt and vitamin B12 deficiency on hematology and liver histology
in sheep with lipidosis.
El-Khodery SA, Hussein HS, Metwally
NS, Nassif MN, El-Boshy
ME, Galbat SA.
Faculty of Veterinary Medicine, Mansoura
University, National research centre,
Doki, Faculty of Veterinary Medicine, Kafe El-Sheikh University, Gyza,
Egypt.
ABSTRACT
El-Khodery SA, Hussein HS, Metwally NS, Nassif MN, El-Boshy ME, Galbat SA, Effect
of cobalt and vitamin B12 deficiency on hematology and liver histology in sheep
with lipidosis, Onl J Vet Res., 28(7): 423-433, 2024. Of 34 ill-thrift anemic sheep,
we found 23 with low serum cobalt 0.24 ± 0.09 mmol/l, vitamin B12 0.15 ±0.07 mg/l and 6 with low vitamin E (1.1
± 0.27mg/l) versus 2.3 ± 0.34 mg/l
in 10 controls. Sheep were examined by ultrasound 5-MHz linear and convex
transducers for liver biopsy microscopy and blood for hematology. We found 5
sheep with mild, 7 moderate and 11 with
severe lipidosis. By ultrasound we detected diffuse echogenicity
and focal hyper-echoic liver parenchyma. Compared with controls, in severe cases
liver size increased ~8% (P < 0.05) whereas in all sheep with the condition,
red cells increased (P < 0.05) 13-24%, hemoglobin 16-23%, packed cell
volumes 17-23% protein 2-18%,
aspartate and alanine transaminases 47-83%. By microscopy we
found hepatic cell vacuolation, congestion of hepatic
sinusoids, biliary hyperplasia and infiltration of portal area with
inflammatory cells and connective tissues.
Key words: Liver, lipidosis, sheep, ultrasonography,
cobalt, microscopy.