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OJVRTM
Online Journal of Veterinary Research©
(Including
Medical and Laboratory Research)
Established 1994
ISSN 1328-925X
25 (10):733-739, 2021.
Avian leukosis virus type J in egg albumin of
layer and broiler flocks by RT-PCR.
M Rajabzadeh1, H Dadras2 and A
Mohammadi3
1Islamic Azad University, Branch of Birjand,
Department(s)
2Avian Medicine, 3Virology, School of Veterinary
Medicine, Shiraz University, Shiraz, Iran.
Rajabzadeh M, Dadras H, Mohammadi
A., Avian leukosis virus subgroup J in egg albumin of
layer and broiler flocks by RT-PCR, Onl J Vet Res., 25 (10):733-739, 2021. Avian leukosis virus
subgroup J (ALV-J) causes mortality and poor performance in broilers. However
there are no treatments or vaccines so prevention of transmission by
identifying infected eggs could control infection. We report detection of ALV-J
in albumen of commercial and native fowl eggs by RT-PCR. Albumen from 370 eggs
from native (70 eggs), broiler grandparent (60 eggs), 3 broiler breeder (180
eggs) and commercial layer flocks (60
eggs) were subjected to RT-PCR RNA ALV-J primers H5/H7 to generate a 545 base
pair fragment. We found ALV-J in 15/180 (8.3%) broiler breeder 17 of 70 (24.3%)
native but none in commercial layers or broiler grandparent farms. Direct
sequencing with specific for subgroup ALV-J primers verified the viral subgroup
in amplification products.
Key words:
Avian Leukosis type J, RT-PCR, egg, albumin.
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