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Online Journal of Veterinary Research©
(Including Medical and Laboratory Research)
25 (10):733-739, 2021.
Avian leukosis virus type J in egg albumin of layer and broiler flocks by RT-PCR.
M Rajabzadeh1, H Dadras2 and A Mohammadi3
1Islamic Azad University, Branch of Birjand, Department(s) 2Avian Medicine, 3Virology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran.
Rajabzadeh M, Dadras H, Mohammadi A., Avian leukosis virus subgroup J in egg albumin of layer and broiler flocks by RT-PCR, Onl J Vet Res., 25 (10):733-739, 2021. Avian leukosis virus subgroup J (ALV-J) causes mortality and poor performance in broilers. However there are no treatments or vaccines so prevention of transmission by identifying infected eggs could control infection. We report detection of ALV-J in albumen of commercial and native fowl eggs by RT-PCR. Albumen from 370 eggs from native (70 eggs), broiler grandparent (60 eggs), 3 broiler breeder (180 eggs) and commercial layer flocks (60 eggs) were subjected to RT-PCR RNA ALV-J primers H5/H7 to generate a 545 base pair fragment. We found ALV-J in 15/180 (8.3%) broiler breeder 17 of 70 (24.3%) native but none in commercial layers or broiler grandparent farms. Direct sequencing with specific for subgroup ALV-J primers verified the viral subgroup in amplification products.
Key words: Avian Leukosis type J, RT-PCR, egg, albumin.