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OJVRTM

Online Journal of Veterinary Research

(Including Medical and Laboratory Research)

Established 1994
ISSN 1328-925X

 

Volume 27 (5): 285-289, 2023.


Lymph node derived Theileria lestoquardi vaccine in sheep.

 

Namavari M1,  Hosseini MH1Esmailnia K2

 

1Razi Vaccine and Serum Research Institute, Shiraz, Iran 2Protozoology Department of Razi Vaccine and Serum Research Institute, Tehran, Iran.

 

ABSTRACT

 

Namavari M,  Hosseini MH, Esmailnia K., Lymph node derived Theileria lestoquardi vaccine in sheep, Onl J Vet Res., 27 (5): 285-289, 2023. Theileria lestoquardi causes malignant theileriosis in sheep and goats. We isolated theileria infected cells from prescapular lymph node of an adult ewe. By PCR we confirmed that ovine lymphocytes were infected by Theileria lestoquardi. DNA was extracted for primers RLB-F2 for Theileria and RLB-R2 for Babesia amplified V4 hypervariable region of 18S rRNA genes. Then 6 animals were inoculated subcutaneously with 5 10⁶ cells infected by T. lestoquardi from 5th culture passage. Controls were 4 sheep injected with normal lymphocytes. Intradermal skin test were done 2 months after infection with freeze-dried Theileria reconstituted antigen by 0.1ml vaccine intradermal injection in infected and controls. Skin thickness was measured by caliper at 72h. We found found no clinical effects. However, in infected sheep we found 1mm thickening by 72h but none in controls. Findings suggested non-attenuated cells did not induce side effects and skin reaction which may have boosted cellular immunity.

 

Keywords: Theileria lestoquardi, sheep, immunization.


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