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Online Journal of Veterinary Research©
(Including Medical and Laboratory Research)
Volume 22 (11):1014-1020, 2018.
Prevalence of Leptospira by PCR in suspect refugees and animals in Iraq.
Hana Abdel Latif Yassin and Hanen Kamel Abbas
Latif Yassin HA, Abbas HK., Prevalence of Leptospira by PCR in suspect refugees and animals in Iraq. Onl J Vet Res., 22 (11):1014-1020, 2018. One hundred and twenty subjects with signs of renal failure, viral hepatitis B or C, or miscarriage were referred from refugee camps in Anbar, Iraq. Sixty serum and urine samples were taken for detection of leptospira by PCR. We also processed 60 serum and 40 urine samples from cattle, sheep and goats suspect of leptospira from veterinary centers. We found only 2/60 positive urine samples in humans, 2/40 in cow urine and 1/60 in bovine blood. By PCR, the 16SrRNA gene bands may not have detected leptospira in human serum but did in urine of two patients with kidney failure. In these individuals the presence of lepstospira may have been due to contact with urine of carrier animals or contaminated streams, ponds, marshes or soils. In cows using PCR 16SrRNA confirmed by another PCR LipL32 gene we found 3 positive for leptospira but none in sheep or goats.
Key-Words:Leptospira, PCR, human, animal, Incidence.