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OJVRTM

 

Online Journal of Veterinary Research

(Including Medical and Laboratory Research)

Established 1994

ISSN 1328-925X

 

Volume 28(3): 167-173, 2024.


Phylogenetic Mycobacterium leprae genome drug targets

 

Saxena A Varadwaj PK, Singh S  Lahiri T.

 

Indian Institute of Information Technology, Allahabad, India Deoghat Jhalwa Campus, RajRooppur Road, alllahabad 211011(U.P.) ( www.iiita.ac.in ), *Corresponding author:  pritish@iiita.ac.in

ABSTRACT

Varadwaj SA,  Lahiri SS., Phylogenetic Mycobacterium leprae genome drug targets, Onl J Vet Res., (3): 167-173, 2024. Reductive evolution filters out pathogen gene set by gene losses to be able to target drug like molecules. Proteins predicted from genome sequence of M. leprae with 2712 FASTA sequences were used to identify horizontally transferred genes by similarity by BLASTP eukaryotes and bacteria in GENBANK wherein proteins scoring 10 E or more were selected. Mycobacteria sequences were removed and absence of bacterial orthologs confirmed by similarity search. Bacterial sequences identified by PSI-BLASTA were aligned using CLUSTALW and subject to phylogenetic analysis, using the neighbor joining and protein parsimony methods of the PHYLIP package.   Non-congruent phylogeny with bootstrapping support => 70 %, were classified as horizontal gene transfer. We identified prolyle tRNA synthetase and uridine phosphorylase in Mycobacterium leprae.

 

KEY-WORDS: Horizontal gene transfer, Computational evolutionary analysis.


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