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OJVRTM
Online Journal of Veterinary Research©
Volume
21(8):563-570, 2016.
Recombinant lentivirus expression vector for insulin-like growth
factor (IGF-1) gene delivery.
Zahra Roudbari1, Mohammadreza Nassiri1, 2*, Mojtaba
Tahmoorespur1, Aliakbar
Haddad-Mashadrizeh2, 3, Ali Javadmanesh1
1Department of Animal Science, Faculty of Agriculture 2Cell
and Molecular Biotechnology Research Group, Institute of Biotechnology, 3Department
of Biology, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, Iran.
*Corresponding Author: Mohammadreza
Nassiri
ABSTRACT
Roudbari Z, Nassiri M, Tahmoorespur M,
Haddad-Mashadrizeh A, Javadmanesh
A., Recombinant lentivirus expression vector for insulin-like growth factor
(IGF-1) gene delivery, Onl J Vet Res., 20(8):563-570, 2016. Insulin-like growth factor gene (IGF1) affects
animal growth. DNA IGF1 was used as transfer vector to
produce recombinant IGF1 in
mammalian cells. Transfer and control vector were co-transfected into HEK293T cell line
with helper plasmids to produce recombinant lentivirus. HEK293T cells with
lentivirus particles were then transducted to produce
recombinant HEK293T cell line. In-vitro mRNA and protein overexpression of
IGF-1 were determined by RT-qPCR and western blotting. The method suggests that a constructed recombinant lentivirus
vector could be used to introduce IGF-1 genes into mammalian cells.
Keyword: IGF1, recombinant lentivirus, growth trait, mammalian
cells.
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