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OJVRTM
Online Journal of Veterinary Research©
Volume 22 (2):150-159, 2007
Electrophoresis of pre and post-natal sheep and goat optic lens protein
Aminlari Ma*, Gholami Sb, Shahsavani
Ea
aDepartment9s) of Biochemistry and bAnatomical
Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran.
ABSTRACT
Aminlari M, Gholami S, Shahsavani E, Electrophoresis
of pre and post-natal sheep and goat optic lens protein, Onl
J Vet Res 22 (2) :150-159, 2018. The electrophoretic
pattern of optic lens protein of sheep and goat at pre- and postnatal ages was
determined. Optic lenses were removed from embryos and postnatal sheep and
goat, homogenized and subjected to sodium dodecyl sulfate polyacrylamide gel
electrophoresis (SDS-PAGE). Electrophoretic patterns of sheep and goat proteins
were similar and the main protein bands were localized in the range of 17-34.4 kDa. With few exceptions, the concentration of proteins was
not affected by sex and age of animals at pre- or postnatal stages. In both
species, a 19.3 kDa protein was dominant and most
concentrated. The concentration of the
20.0 and 34.4 kDa proteins gradually decreased and
several low molecular weight proteins (less than 17 kDa)
appeared at postnatal ages, suggesting increased proteolysis of lens protein
with age. The results indicate that optic lens proteins of sheep and goat are
similar to each other and very similar to the published proteins of other
species, particularly cattle, but distinctively different from those of camel
and chicken.
Key words:
Sheep; Goat, Crystallins; Optic lens; SDS-PAGE.
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