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OJVR
Online Journal of Veterinary Research©
Volume 14 (1):75-86, 2010. Redacted
2017.
Genetic
diversity studies of long-term selected White Leghorn strains vis a vis a brown
egger population by multi-locus DNA (RAPD) markers
Mahadeo Kumar1, SK Mishra2, VLK Prasad3, RP
Sharma4 and BR Gupta5.
1Mahadeo Kumar, M.V.Sc., Scientist, Indian Institute of Toxicology Research,
Lucknow 2 S.K. Mishra, Ph.D., Senior Scientist, Central Avian Research Institute, Izatnagar, Bareilly- 243122. 3 V.L.K Prasad, Ph.D., Professor,
Department of Livestock Production and
Management, College of Veterinary Science, Rajendranagar
Hyderabad-500030 (A.P.), 4 R. P. Sharma, Ph.D., Director, Project Directorate on Poultry,
Hyderabad, India- 500030.5 B.R Gupta, Ph.D., Department
of Animal Genetics and Breeding, College of Veterinary Science, Hyderabad-500030
(A.P.)
ABSTRACT
Kumar M, Mishra
SK, Prasad VLK, Sharma RP, Gupta BR, Genetic diversity studies of long-term
selected White Leghorn strains vis a vis a brown egger population by
multi-locus DNA (RAPD) markers, Onl J Vet Res, 14 (1):75-86, 2010. We analyzed
genetic diversity of 5 egg-type closed chicken populations, viz. 4 White
Leghorn (WLH) strains: IWI, IWH, IWF and IWD which had undergone selection for
more than 20 generations, and one brown-egger strain: D. Red with variant
geographic origin. A cumulating total of 145 randomly amplified polymorphic DNA
(RAPD) markers generated from 10 most polymorphic primers, screened out of 42,
were employed. The WLH strains exhibited distinct inter-population genetic
differences as correlated to their origin and subsequent selection history. The
intra-population genetic similarity ranged from 0.8484 to 0.9011 whereas inter-population
genetic similarity ranged 0.5682 to 0.8854.
The coefficient of population-genetic differentiation (GST)
was 0.422 which indicated higher contribution of within-population
differences to genetic variation. A combined Dendrogram
incorporating all the above strains in form of un-rooted tree was constructed,
which revealed that the D. Red clustered distinctly away from the WLH strains, while
displaying the highest genetic distance from IWD (0.5645). Within the WLH
strains, the IWI and IWD were at maximum genetic distance (0.23) while the least
(0.122) distance was recorded between IWF and IWD. It was concluded that
differential genetic-homogeneity and distinct clustering patterns within egg
type chicken strains consistent to their breeding and selection histories could
be efficiently delineated by RAPD markers.
Key words: Genetic diversity,
polymorphism, RAPD, Long-term selection and WLH.
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