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OJVRTM
Online Journal of Veterinary Research©
Volume
23 (1):75-88, 2019.
Effect of light emitting diode (LED) irradiation on bovine In Vitro embryo growth.
Luis E. Vergara DMV Esp
MSc1 , Mateus J. Sudano DMV Esp
MSc PhD1, Jefferson V. Diniz
DMV Esp MSc PhD*1, Pablo E. Ocampo-Ortiz
DMV MSc1,
Andrés C. Moreno-Guio
MV 2. Mauricio
Montoya-Flórez1,3 DMV MSc PhD2,
Fernanda da Cruz Landim DMV
Esp MSc PhD1.
1Faculty of Veterinary Medicine, São Paulo State
University-UNESP, Botucatu, distrito de Rubião Jr Botucatu, SP 18618-000. 2Pedagogical and Technological University of
Colombia, Tunja
3Colômbia Research Group in Veterinary
Medicine and Husbandry - GIDIMEVETZ, Pedagogical and Technological University
of Colombia, Tunja, Grupo de Pesquisa em Patologia Veterinária, Faculdade de Ciências
Agropecuarias, Universidade de Caldas Manizales, COL. Faculty of Veterinary Medicine, Universidad Nacional de Colombia, Bogotá
COL.*Corrresponding
author: jeffvianadiniz@gmail.com, maomontoya53@yahoo.es
ABSTRACT
Vergara LE, Sudano
MJ, Diniz JD, Ocampo PE,
Moreno-Guio A, Montoya-Flórez
, Landim F. Effect of light emitting diode (LED) irradiation on In Vitro
bovine embryos, Onl J Vet Res. 23 (1):75-88, 2019. Different tools have been
proposed in order to increase in vitro
bovine embryo production which remains low and sub lethal stressing to
stimulate embryonic cells has been proposed. We describe effect of sub-lethal
irradiation (LED) on bovine in vitro
embryo production. One hundred and fifty zygotes were divided into 9 groups of 1
control, 4 irradiated with wavelengths of 633 nm (Red) and 4 with 820 nm
(Infrared) in dosages equivalent to 10 J/cm². At different points, half of the
zygotes underwent analysis through TUNEL assay to identify DNA fragmentation rates while the other
half was frozen, thawed and submitted to the same analysis. Data was analyzed
by ANOVA followed by Tukey’s test (P<0.05).
We could not demonstrate a direct impact of LED sublethal
stress over embryonic growth but in groups irradiated with infrared light we
found a degree of protection for embryonic cells without affecting blastocyst
production rate. Further studies with LED should attempt other wavelengths,
filters, dosages and/or exposure times.
Key Words: DNA,
irradiation, LED, sublethal stress, zygote.
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