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Online Journal of Veterinary Research©
Volume 22 (6):434-443, 2018.
Effect of lead with or without thiamine and calcium EDTA on hematology in young dogs.
1Maher Saber Owain and 1Afaf Abdulrahman Yousif
1Department of Internal and Preventive Veterinary Medicine, College of Veterinary Medicine, University of Baghdad, Iraq.
Owain MS, AA Yousif., Effect of lead with or without thiamine and calcium EDTA on hematology in young dogs. Onl J Vet Res 22(6):434-443, 2018. Lead can induce toxicity especially in young animals and children. We report effect of lead drench with or without thiamine or calcium EDTA on hematological markers in young pound dogs. Groups of five 2-4 month old mixed breed dogs each were drenched 15 mg/kg/day lead acetate for 60 days (lead controls) and then treated (at 60 days) with or without 110mg/kg calcium EDTA twice daily subcutaneously (SC) for three days, or 25mg/kg thiamine SC daily for one week. Five controls were left untreated. Five ml blood was collected in EDTA tube for hematology at days 0, 15, 30, 45 60 and 75. We found declines (P < 0.05) at 60 days in PCV, hemoglobin, red blood cells count, mean corpuscular volume and mean corpuscular hemoglobin. Blood smears exhibited rouleaux formation and hypochromic erythrocytes with many immature erythrocytes with basophilic stippling, hypersegmented neutrophils, acanthocytes and anisocytosis. By day 45 of lead drench differential leukocyte and neutrophils count increased and lymphocytes decreased (P < 0.05). In dogs given lead at day 60 with EDTA this continued to day 75 whereas in those given lead with thiamine or Calcium EDTA hematological values returned to normal by day 75. We find that lead affected all hematological parameters in dogs but that concurrent thiamine-Calcium EDTA reduced toxic effects compared with other treatments and controls by day 75.
Key words: Puppies, lead, thiamine, Ca-EDTA, Lead.